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IEX-MALS – A method for protein separation and characterization

Waters | Wyatt Technology
7 Jan 2025

Ion-exchange chromatography coupled to multi-angle light scattering (IEX-MALS) allows absolute molar mass determination of proteins, protein complexes and peptides in a heterogenous sample. IEX-MALS can overcome some of the restrictions of SEC-MALS that arise from the limited separation ability of analytical SEC column. Explore how the IEX-MALS method is valuable for quality assessment as well as the characterization of native oligomers, charge variants, modified proteins and mixed-protein samples.

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miniDAWN™

Waters | Wyatt Technology

The miniDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering superb sensitivity over a wide range of molecular weight, size and concentration.

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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MALS

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IEX-MALS- a method for protein separation and characterization