Use case: His-tag protein expression screening from crude lysates

10 Nov 2025

His-tagged proteins underpin work across structural biology, assay development, and drug discovery. When screening many constructs, teams need quick, quantitative readouts from crude lysates to decide what to scale and what to drop.

In this application note, Abselion demonstrates how by using a His-tag assay on the Amperia™ platform, teams can obtain reproducible concentration measurements directly from unpurified samples, informing expression and purification decisions earlier.

Amperia™ Protein Quantification System

Abselion

The Amperia™ system combines compact hardware, assay-ready sensors, and integrated software to streamline protein quantification. Built to support scientists in discovery, development, and beyond, it delivers dependable data quickly and without unnecessary complexity — even from crude or complex samples.

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Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.