ResourceDrug Discovery & Development
High-throughput cytometry for MSC, iPSC, and CAR-T studies
Learn how you can transform cell therapy screening workflows at all stages of research
22 May 2025
Cameron Smith-Craig
Pharma and Applied Sciences Editor
From regenerative medicine to cancer immunotherapy, the promise of cell therapies continues to expand. However, there are many challenges on the path from research to clinical application – particularly amongst analysis. One of the primary frustrations for researchers today is the complexity of advanced cell models, coupled with the need for efficient and scalable workflows that they can use to screen, characterize and monitor therapeutic cells.
This application guide highlights the use of High-Throughput Screening (HTS) by Cytometry to combat these challenges, particularly for mesenchymal stem cell (MSC), induced pluripotent stem cells (iPSC) and CAR-T studies.
Key topics explored:
- Mesenchymal stem cell identity and differentiation
- Optimizing advanced cell models
- Characterization and optimization of iPSCs
- Phenotypic and functional characterization of CAR-T cells
- Characterization of T cell activation
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow CytometryMesenchymal Stem CellsPluripotent Stem CellsCAR-T cell therapyCell TherapyCell therapy involves using living cells to treat diseases, often by replacing damaged cells or stimulating regeneration. Stem cell therapy and CAR-T cell therapy are examples of cutting-edge treatments in regenerative medicine and cancer immunotherapy. Browse our peer-reviewed product directory to find the best cell therapy tools, compare products, check reviews, and get pricing directly from manufacturers.