Eppendorf BioBLU® Single-use Vessels
Eppendorf BioBLU Single-use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design.
BioBLU c (cell culture)
BioBLU f (fermentation)
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Eppendorf BioBLU Single-use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design.
Single-use vessels eliminate autoclaving, improve turn-around time, simplify validation, and reduce overall costs. The BioBLU portfolio has grown to accommodate users from early research and development through manufacturing, across a wide variety of processes, including shear sensitive cell cultures, robust microbial applications, and adherent cell line development.
The BioBLU 0.3f and BioBLU 1f Single-use Vessels allow microbial process development at the highest level. These rigid wall stirred-tank single-use vessels have been specifically designed for high cell density fermentation with bacteria, fungi and yeast. BioBLU f vessels can be used with the Eppendorf DASbox® and DASGIP® Parallel Bioreactor Systems.
Compared to cell culture applications, fermentation processes require much higher kLa values for proper mass transfer as well as suitable heating and cooling options. Proven stirred-tank design, powerful overhead drives featuring Rushton-type impellers and innovative baffles for cooling make the BioBLU f vessels achieve these demands.
Eppendorf BioBLU® Single-use Vessels Features:
- Market leading portfolio of single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 65 mL to 40 L working volumes.
- All BioBLU c vessel sizes available with pitched blade impellers for cell culture applications.
- BioBLU 5p vessels with packed-bed impeller, pre-loaded with Fibra-Cel® disks.
- BioBLU 0.3f and 1f vessels with Rushton-type impellers for microbial applications.
- Unique non-invasive pH and DO sensor technology drastically reduces contamination risks (industry standard autoclavable pH sensors available for pH measurement).
- Sealed magnetic drive with fully enclosed bearings maintain vessel sterility.
- Raw materials used to produce BioBLU vessels comply with USP Class VI standards and are BSE/TSE free.
- Vessels are assembled with sparger, overlay, and exhaust filters as well as penetrations for pH, DO, temperature, liquid additions, sampling and harvest.
- For use with DASbox Mini Bioreactor System, DASGIP Parallel Bioreactor Systems and New Brunswick CelliGen BLU controllers.
- Adaptor kits for legacy and 3rd party bioreactor controllers available (BioBLU 1, 5 and 14, and 50).
- Validation packages available upon request.
Applications:
- Insect, mammalian and human suspension cell lines, and stem cells Adherent cells Bacteria, yeasts and fungi.
- Adherent cells.
- Bacteria, yeasts and fungi.
Transforming bioprocess data into actionable intelligence
Managing the weight of bioprocessing data can often overwhelm laboratories, but effective data management is both achievable and critical to successful biopharmaceutical development.
This application eBook highlights advanced instruments and digital tools that integrate seamlessly into laboratory infrastructure, enabling continuous monitoring and streamlined data management. With remote accessibility, these solutions empower scientists to maintain oversight and control of bioprocessing data beyond the physical lab environment.
Download to learn:
- How to conduct accurate and efficient real-time monitoring of Chinese hamster ovarian (CHO) cultivation using the BioNsight®
- Case studies using automated technologies for sampling CHO and E.coli cultures to understand growth conditions, and improve experimental throughput.
- The potential of Raman spectroscopy to accurately track critical process parameters
Biocompatibility studies on CHO cell culture in X-ray irradiated BioBLU Single-Use Bioreactors
The potential leakage of toxic or inhibitory chemicals from plastic materials from single-use bioreactors is increasingly known within cell culture. Leachables are particularly problematic, as they can migrate from the plastic into the process fluid under process conditions and can inhibit cell growth or affect the final product quality. By strictly adopting the 'DECHEMA Recommendation for Leachables Studies – Standardized cell culture test for the early identification of critical films', Eppendorf demonstrates the absence of negative impacts caused by leachable compounds from the X-ray irradiated material on growth, viability, and metabolic profile of the CHO cell line tested.
Optimizing cell and gene therapy workflows
In this guide, Eppendorf provides detailed information on optimizing cell and gene therapy workflows and options for scaling up cell cultures with advanced bioreactor systems. Cell and gene therapies are emerging fields of transformative medicine where diseases are treated by restoring or reconditioning cells or genes. Cells and genetically engineered cells demonstrate vastly different properties than traditional medicines and surgery. Akin to “living drugs”, they can heal and replace diseased organs and thus have the potential to provide curative therapies for various diseases.
Harnessing the latest bioprocessing technology in cell and gene therapy
Discover how the latest innovations in upstream bioprocessing are serving the cell and gene therapy industry
