Extraction of Permethrin Pesticides from Spinach Using QuEChERS Methodology with Automated Shaking

27 Nov 2015

QuEChERS was introduced in 2003 for simplified sample preparation in the analysis of pesticides in fruits and vegetables. In this article, permethrin was extracted from spinach using the Supel™ QuE 50 mL centrifuge tubes and following the QuEChERS procedure. Analysis was performed on capillary GC column SLB®-5ms with MS detection.

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Supel™ QuE
Sigma-Aldrich Supelco
Dispersive SPE (dSPE), often referred to as the "QuEChERS" method (Quick, Easy, Cheap, Effective, Rugged, and Safe), is modern sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products. Using the QuEChERS method, food/agricultural samples are first extracted with an aqueous miscible solvent (e.g., acetonitrile) in the presence of high amounts of salts (e.g., sodium chloride and magnesium sulfate) and/or buffering agents (e.g. citrate) to induce liquid phase separation and stabilize acid and base labile pesticides, respectively. Upon shaking and centrifugation, an aliquot of the organic phase is subjected to further cleanup using SPE. Unlike traditional methods using SPE tubes, in dispersive SPE, cleanup is facilitated by mixing bulk amounts of SPE (e.g., Supelclean PSA, ENVI-Carb, and/or Discovery DSC-18) with the extract. After sample cleanup, the mixture is centrifuged and the resulting supernatant can either be analyzed directly or can be subjected to minor further treatment before analysis. Supelco carries a line of vials and centrifuge tubes containing pre-determined amounts of salts and SPE sorbents to support the most common method configurations used today.
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SLB-5ms Capillary GC Column
Sigma-Aldrich Supelco
SLB-5ms Capillary GC Column Application: The 5% phenyl equivalent phase provides a boiling point elution order with a slight increase in selectivity, especially for aromatic compounds. The low bleed characteristics, inertness, and durable nature make it the column of choice for environmental analytes (such as semivolatiles, pesticides, PCBs, and herbicides) or anywhere a low bleed non-polar column is required. USP Code: This column meets USP G27 and G36 requirements. Phase: Bonded and highly crosslinked; silphenylene polymer virtually equivalent in polarity to poly(5% diphenyl/95% dimethyl siloxane) Temp. Limits: ≤0.32 mm I.D.: -60 °C to 340 °C (isothermal) or 360 °C (programmed) Temp. Limits: ≥0.53 mm I.D.: -60 °C to 330 °C (isothermal) or 340 °C (programmed)
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Supel™ QuE Empty Centrifuge Tube with Lid
Sigma-Aldrich Supelco
“QuEChERS” (Quick, Easy, Cheap, Effective, Rugged, and Safe) - Supel™ QuEEmpty Centrifuge Tube with Lid, centrifuge tube volume 50 mL, pk of 50, suitable for EN 15662:2008 per BSDispersive SPE (dSPE), often referred to as the "QuEChERS" method, is an emerging sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products. In dSPE, food/agricultural samples are first extracted with aqueous miscible solvents in the presence of high amounts of salt and/or buffering agents followed by SPE clean up. Unlike traditional methods that use SPE tubes, in dSPE clean up is facilitated by mixing bulk amounts of SPE with the extract. Supelco now carries a line of centrifuge tubes containing pre-determined amounts of salts and SPE sorbents to support the most common dispersive SPE methods used today.Supelco offers vials and centrifuge tubes in bulk quantities, each packed with pre-determined amounts of salts and SPE sorbents to support the most common method configurations used today. This saves the analyst the time taken to weigh out the individual materials multiple times.Our Dispersive SPE Customization Service features:• 50 mL centrifuge tubes• 12 mL centrifuge tubes• 2 mL microcentrifuge tubes• Glass vials• A wide selection of Sigma-Aldrich/Supelco salts, reagents, and SPE phases
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Supel™ QuE PSA/C18 Tube
Sigma-Aldrich Supelco
“QuEChERS” (Quick, Easy, Cheap, Effective, Rugged, and Safe) - Supel™ QuEPSA/C18 Tube, pack of 100, suitable for 2007.01 per AOACDispersive SPE (dSPE), often referred to as the "QuEChERS" method, is an emerging sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products. In dSPE, food/agricultural samples are first extracted with aqueous miscible solvents in the presence of high amounts of salt and/or buffering agents followed by SPE clean up. Unlike traditional methods that use SPE tubes, in dSPE clean up is facilitated by mixing bulk amounts of SPE with the extract. Supelco now carries a line of centrifuge tubes containing pre-determined amounts of salts and SPE sorbents to support the most common dispersive SPE methods used today.Supelco offers vials and centrifuge tubes in bulk quantities, each packed with pre-determined amounts of salts and SPE sorbents to support the most common method configurations used today. This saves the analyst the time taken to weigh out the individual materials multiple times.Our Dispersive SPE Customization Service features:• 50 mL centrifuge tubes• 12 mL centrifuge tubes• 2 mL microcentrifuge tubes• Glass vials• A wide selection of Sigma-Aldrich/Supelco salts, reagents, and SPE phases
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Supel™ QuE Acetate Tube
Sigma-Aldrich Supelco
"QuEChERS” (Quick, Easy, Cheap, Effective, Rugged, and Safe) - Supel™ QuE Acetate Tube, pk of 50, suitable for 2007.01 per AOACDispersive SPE (dSPE), often referred to as the "QuEChERS" method, is an emerging sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products. In dSPE, food/agricultural samples are first extracted with aqueous miscible solvents in the presence of high amounts of salt and/or buffering agents followed by SPE clean up. Unlike traditional methods that use SPE tubes, in dSPE clean up is facilitated by mixing bulk amounts of SPE with the extract. Supelco now carries a line of centrifuge tubes containing pre-determined amounts of salts and SPE sorbents to support the most common dispersive SPE methods used today.Supelco offers vials and centrifuge tubes in bulk quantities, each packed with pre-determined amounts of salts and SPE sorbents to support the most common method configurations used today. This saves the analyst the time taken to weigh out the individual materials multiple times.Our Dispersive SPE Customization Service features:• 50 mL centrifuge tubes• 12 mL centrifuge tubes• 2 mL microcentrifuge tubes• Glass vials• A wide selection of Sigma-Aldrich/Supelco salts, reagents, and SPE phases
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