Effectiveness of CRISPR-Cas9 Using Pools of Synthetic crRNAs in High-Content Analysis Screening Experiments
3 Jan 2019Gene knockout using the CRISPR-Cas9 system has emerged as a powerful technology for loss-of-function screening. Although screening using pooled lentiviral sgRNA libraries is a powerful way of discovering gene function arrayed screening expands the types of phenotypic readouts from simple population enrichment or depletion to complex multiparametric high-content imaging and morphological assays. Chemical synthesis of guide RNAs for CRISPR-Cas9 gene editing allows for accurate and rapid production of CRISPR libraries and enables screening in an arrayed, one-gene-per-well fashion.
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High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.GenomicsGenomics is the study of genomes, focusing on the sequencing, analysis, and interpretation of genetic material. It is key in understanding genetic diseases, evolutionary biology, and personalized medicine. Techniques like next-generation sequencing (NGS) are commonly used in genomics research. Browse our peer-reviewed product directory to find the best genomics tools, compare products, check reviews, and get pricing directly from manufacturers.ScreeningUsing robotics, data processing and control software, liquid handling devices and sensitive detectors, screening allows a researcher to quickly conduct millions of chemical, genetic or pharmacological tests.CRISPRCRISPR technology enables precise editing of genes, allowing scientists to modify DNA at specific locations. This revolutionary tool is used in genetic research, drug development, and gene therapy. CRISPR has applications in agriculture, disease treatment, and creating genetically modified organisms (GMOs). Explore CRISPR solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.