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Direct measurements of cellular metabolism for identification of mitochondrial drug targets

Agilent Technologies
31 Jul 2023

In this application note, Agilent Technologies discusses the importance of drug target identification in drug discovery. Phenotypic screening is one method used, involving the addition of compounds to cells to observe their impact on cell activity. Agilent Seahorse XFe96 Analyzer is introduced as an ideal system for measuring mitochondrial respiration and cell metabolism in live cells, providing insights into metabolic drug targets. The workflow includes the Seahorse XF Cell Mito Stress Test to assess the compound's effect on mitochondrial function and subsequent XF assays with permeabilized cells to identify specific targets. Incorporating direct cell-based measurements offers valuable insights into compound effects and metabolic modulation for drug target identification.

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Seahorse XF Plasma Membrane Permeabilizer

Agilent Technologies

The Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) is a proprietary reagent that permeabilizes intact cells in culture. It selectively targets the cellular plasma membrane while leaving the mitochondrial membrane intact. This selectivity enables the detailed characterization of key components in mitochondrial function including transporters, enzymes, and electron transport chain complexes.

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Combinatorial ChemistryCombinatorial chemistry, also known as combichem, is a technique used in drug discovery to create libraries of structurally related compounds. A library is generated by synthesis with a chemical reactor system or by computer-based modeling of compound combinations. When undertaking combinatorial chemistry consider reagents, buffers, resins and standards.Drug DiscoveryDrug discovery is the process of identifying potential new medications, involving stages such as target identification, compound screening, and preclinical development. It relies on cutting-edge technologies like high-throughput screening, artificial intelligence, and molecular modeling to accelerate the identification of drug candidates. Drug discovery plays a pivotal role in developing new therapies for diseases ranging from cancer to rare genetic disorders. Browse our peer-reviewed product directory to find the latest drug discovery technologies, compare options, check customer feedback, and get pricing directly from manufacturers.PharmaceuticalsPharmaceuticals are medicinal drugs used in healthcare to diagnose, prevent, cure and treat illnesses. Pharmaceuticals that are excreted after use appear in wastewater and can have detrimental effects on the environment.Phenotypic ScreeningPhenotypic screening assesses cellular responses to compounds, enabling drug discovery and target identification. This technique is pivotal in finding effective treatments for complex diseases. Discover phenotypic screening platforms and tools with peer-reviewed comparisons and pricing in our directory.Drug DevelopmentDrug development refers to the process of bringing a new drug to market.

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Direct measurements of cellular metabolism for identification of mitochondrial drug targets