Seahorse XF Plasma Membrane Permeabilizer
The Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) is a proprietary reagent that permeabilizes intact cells in culture. It selectively targets the cellular plasma membrane while leaving the mitochondrial membrane intact. This selectivity enables the detailed characterization of key components in mitochondrial function including transporters, enzymes, and electron transport chain complexes.
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The Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) is a proprietary reagent that permeabilizes intact cells in culture. It selectively targets the cellular plasma membrane while leaving the mitochondrial membrane intact. This selectivity enables the detailed characterization of key components in mitochondrial function including transporters, enzymes, and electron transport chain complexes.
Features
- Selectively targets the cellular plasma membrane for permeabilization
- Study mitochondrial function without isolating mitochondria
- 1.0 nM works for most cell types, meaning that minimal optimization is required
- The XF PMP reagent provides consistent results in every assay
Direct measurements of cellular metabolism for identification of mitochondrial drug targets
In this application note, Agilent Technologies discusses the importance of drug target identification in drug discovery. Phenotypic screening is one method used, involving the addition of compounds to cells to observe their impact on cell activity. Agilent Seahorse XFe96 Analyzer is introduced as an ideal system for measuring mitochondrial respiration and cell metabolism in live cells, providing insights into metabolic drug targets. The workflow includes the Seahorse XF Cell Mito Stress Test to assess the compound's effect on mitochondrial function and subsequent XF assays with permeabilized cells to identify specific targets. Incorporating direct cell-based measurements offers valuable insights into compound effects and metabolic modulation for drug target identification.
Agilent Seahorse XF assay kits and reagents
In this application note, Agilent Technologies offers a wide range of Seahorse XF assay kits, reagents, and media for studying cellular function, including metabolism and energetics, and assessing mitochondrial and glycolytic function in real time. These kits are designed to simplify workflows and improve research productivity, enabling consistent data generation on the Seahorse XF analyzer.
Agilent Seahorse XF plasma membrane permeabilizer
In this application note, Agilent Technologies introduces the Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP), a cell permeabilization reagent. It offers easy access to mitochondria without the need for detergents, beads, or gradients. Most cell types work at 1.0 nM with minimal optimization, and the mitochondrial membrane remains undamaged for consistent assay results. The proprietary reagent selectively targets the cellular plasma membrane, enabling researchers to measure mitochondrial dysfunction without isolating mitochondria. Seahorse XF PMP is compatible with Agilent Seahorse XFe and XF Analyzers, providing valuable information that can be difficult to obtain with intact cells or isolated mitochondria.
