Detect and modulate human T cell activation using the Agilent Seahorse XF Hu T Cell Activation Assay Kit
31 Jul 2023In this application note, Agilent technologies presents a novel approach to detect and modulate human T cell activation using the Seahorse XF Hu T Cell Activation Assay. This rapid assay measures proton efflux rates (PER) associated with glycolytic pathway-dependent energy production, offering real-time observation and early detection of T cell activation. The assay is supported by Seahorse Analytics software for data analysis, and the kit includes validated reagents and protocols for robust results.
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Seahorse XF Hu T Cell Activation Assay Kit
Agilent TechnologiesAgilent Seahorse XF Hu T Cell Activation Assay Kit measures human (Hu) T cell activation response within several minutes of stimulation using Seahorse XF Analyzers. This is accomplished via measuring proton efflux rates (PER) associated with glycolytic pathway-dependent energy production, and is built on the principle that T cell activation is accompanied by a rapid switch in cellular energy production and intermediate demand to support effective proliferation, size expansion, and differentiati