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Characterization of hyaluronic acid with online multi-angle light scattering and differential viscometry
3 Feb 2025The biopolymer hyaluronic acid is present in all mammalian systems and is of enormous commercial interest for opthalmic, medical, pharmacological and cosmetic applications. In this application note, the absolute determination of hyaluronic acids' properties is demonstrated using MALS, differential refractometry, and differential viscometry detectors in series with SEC separation.
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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ViscometersViscometers are used to measure the viscosity of a fluid. Types of viscometer include rotational, vibrational and falling piston viscometers. Options for viscometers include modular, portable, benchtop and temperature compensation and density measurement features.Multi Angle Light Scattering