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Automated 3D cell culture and drug testing using Corning Matrigel Matrix

Tecan
1 Jan 2025

This application note details an automated workflow for 3D cell culture. It includes controlled pipetting of Matrigel® matrix, cell seeding and spheroid cultivation using a Fluent Automation Workstation with an integrated, liquid-cooled four-position carrier and a Thermo Scientific™ Cytomat™ 2 C450-LiN automated incubator. Drug treatments were performed using the D300e Digital Dispenser. A Spark® Cyto multimode reader was used to monitor cell growth by brightfield imaging, as well as to perform luminescence-based viability assessment using the CellTiter-Glo® 3D Cell Viability Assay (Promega).

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Fluent® Laboratory Automation Workstation

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Spark® Cyto

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Spark Cyto is the first live cell plate reader to detect biological, chemical or physical events – capturing the maximum amount of data possible from each plate well, at the same time and under the same conditions.

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D300e Digital Dispenser

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The D300e Digital Dispenser* uses direct digital dispensing technology to enable rapid delivery of picoliter to microliter sample volumes. It delivers fast, reliable performance across a large dynamic range, eliminating the need for slow, error-prone and wasteful serial dilutions for creation of dose-response curves and enabling miniaturized qPCR reaction set-up.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.3D Cell CultureCulturing cells in three dimensions allows more physiologically relevant observations to be made regarding cellular interactions and gene expression. 3D models provide a more accurate representation of cells in vivo and are more stable than 2D cultures, making them very useful for long-term studies.OrganoidsSpheroidsSpheroids are clusters of cells that have been grown in 3D culture to be used as <i>in vitro</i> model systems. These 3D microtissues can be used for toxicology testing, DMPK studies and many other applications involving cell analysis.

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