AOAC-RI PTM and NF validation of the Thermo Scientific Listeria Species PCR Assay

24 Sept 2020

This poster outlines AOAC-RI PTM and NF VALIDATION studies that demonstrate that the Thermo Scientific Listeria Species PCR workflow, including the QuantStudio 5 Food Safety System, is a suitable method for the detection of Listeria species in a broad range of foods, with superior or equivalent performance to the reference method.

SimpliAmp™ Thermal Cycler

Applied Biosystems (Europe)

The SimpliAmp™ Thermal Cycler is a small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR. It is crafted with the reliable engineering and performance you’ve come to expect from Applied Biosystem® instruments. It is elegantly designed to deliver quiet cycling, and features an innovative, safe heated lid and a large LCD color touch screen. Key Features and Benefits: VeriFlex™ blocks to optimize PCR temperature with precision—the VeriFlex™ blocks provide 3 independent temperature zones, for precise temperature control over your PCR optimization. 8-inch color touch screen to help save time—the large and responsive color touch screen simplifies instrument operations with an easy to read interface. WiFi-enabled for remote monitoring—conveniently monitor your instrument from anywhere with our free PCR Essentials mobile app. Simple lid design to protect your sample—the innovative heated lid is easy to use and designed to help protect your samples. When open, it is in an upright position for easy handle access. Pull the handle forward to close the lid with ease. The heated lid will open automatically if not closed properly, to help safeguard your precious sample. Ready to use USB drive to easily back up your data—simply plug in a USB drive and follow the on-screen prompts to back up your data simply and effortlessly. Thermal simulation modes to replicate other PCR experiments—a library of simulation modes is available so that you may run an experiment using the same ramp rates as with a previous or different instrument.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food and Beverage ProcessingFood and Beverage Processing involves the preparation of food products for further analysis. Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.Food SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Food ContaminationFood contamination refers to the presence of harmful substances or pathogens in food, posing risks to human health. Contaminants can be biological, chemical, or physical, and their detection is crucial for ensuring food safety. Advanced testing methods like PCR, chromatography, and spectroscopy are used to identify contaminants in food products. Browse our peer-reviewed product directory to find the best solutions for food contamination detection, compare products, check reviews, and get pricing directly from manufacturers.Food QualityFood quality refers to the attributes of food that influence its safety, nutrition, and taste. Monitoring and ensuring food quality are essential in production to meet health regulations and consumer expectations. Techniques like spectroscopy, chromatography, and microbial testing are used to assess food properties and detect contaminants. Browse our peer-reviewed product directory to find food quality testing solutions; compare products, check reviews, and get pricing directly from manufacturers.