A High Throughput Drug Discovery for Intracellular Neurodegenerative Disease-Associated Targets using the LI-COR® Odyssey® Near Infrared Imaging System
11 Jan 2016Intracellular abnormal tau accumulation has been indicated in Alzheimer's disease (AD) progression. The LI-COR® Odyssey® scanner has been used to develop a novel, 96-well cell-based assay suited for high throughput detection of changes in tau protein levels. The dual near-infrared detection capability of the LI-COR® Odyssey® scanner allows for simultaneous first-pass estimations of toxicity by measuring glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels within the same well. Using this system, we have identified a panel of novel HSP90 inhibitors with potent tau-reducing activity.
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High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Alzheimers ResearchAlzheimer�s disease is a neurodegenerative condition characterized by progressive memory loss and cognitive decline. Research focuses on understanding the molecular and cellular mechanisms of the disease, as well as developing effective treatments and diagnostic tools. Browse our peer-reviewed product directory to find the best Alzheimer�s research products, compare options, check reviews, and get pricing directly from manufacturers.Targeted ScreeningCompound ScreeningCompound screening is a method used to discover specific compounds that could be promising candidates for pharmaceutical use. This potential is identified when compounds interact with the target protein during screening and could therefore be carried forward in the drug development process.Cell CultureCell culture involves growing cells under controlled conditions, typically outside of their natural environment, to study cellular behavior, drug response, and disease models. Cell culture is crucial in biotechnology and biomedical research. Explore cell culture products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.NeuroscienceNeuroscience research investigates the neurological mechanisms underlying behavior, neurodegenerative diseases, and other brain conditions. Learn about the innovative technologies for bioimaging, electrophysiology, cell culture, chromatography and other techniques used in this field.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.