A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-Line Enrichment and Class Specific Separation of Phospholipids

23 Jan 2014

Lipidomics is a blossoming field of study for identifying biomarkers and potential drug targets in cancer research. In this application note, learn how Waters UPLC and tandem quadrupole MS technologies deliver a highly productive workflow from extraction to separation, identification and quantification of phospholipids

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ACQUITY UPLC System
Waters
Waters ACQUITY UPLC - Dramatic Improvements in Speed, Sensitivity and Resolution Whether you work in methods development or quality assurance, in drug discovery and development or testing for safety in food supplies, you seek a productivity edge. With the ACQUITY UPLC® System, now you can get more information in a single, short run than you've ever seen with HPLC. Waters ACQUITY UPLC System UltraPerformance LC® (UPLC®) technology starts with unique 1.7 μm small-particle chemistries. Chromatographers no longer need to choose between speed and resolution–with UPLC you get both. Instrument Designed for Chemistry; Chemistry Designed for the Instrument The ACQUITY UPLC System has been holistically designed to match the performance needs of our innovative column chemistries with robust hardware, easy-to-use software and specialized support services. Small, pressure-tolerant particles High-pressure fluidic modules Minimized system volume Negligible carryover Reduced cycle times Fast response detectors Integrated system software and diagnostics With UPLC you have the ability to work more efficiently with higher speed, sensitivity and resolution at a much wider range of linear velocities, flow rates and backpressures to obtain superior results. The Waters ACQUITY UPLC System's high-pressure fluidics optimizes flow rates to make the most of small particle technology. The ACQUITY UPLC System's sample-handling design is designed to ensure exceptionally low carryover and reduced cycle time. And when interfaced with the Sample Organizer, it increases unattended sample capacity by ten times. High-speed detectors, both optical and mass, contribute to increased sensitivity and help manage the heightened speed and resolution requirements of UPLC. ACQUITY UPLC Systems are easily controlled, diagnosed, and monitored via a graphical system console interface. The console offers: Quick and easy access to critical instrument parameters Simple system start-up, elegant system status monitoring and predictive performance indicators to ensure maximum productivity Data management capabilities that are supported by both MassLynx™ and Empower™ software The ACQUITY UPLC System is also supported by Intelligent Device Management technology with our Connections® INSIGHT™ service, providing instrument diagnostics.
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ACQUITY UPLC® BEH HILIC Columns
Waters
ACQUITY UPLC® BEH HILIC columns contain the rugged 1.7 μm unbonded BEH particles and are designed to retain and separate very polar basic compounds.These unique columns are optimized and tested to produce efficient and reproducible separations under UPLC® HILIC conditions. ACQUITY UPLC BEH HILIC columns overcome a major weakness of HILIC stationary phases: chemical stability. The unbonded silica stationary phases often used in HILIC are prone to dissolution at neutral pH. The rugged BEH particle’s wide usable pH range overcomes this pH limitation and results in long column lifetimes. ACQUITY UPLC™ BEH HILIC columns offer advantages such as retention and separation of very polar basic compounds, alternate selectivity as compared to reversed-phase chromatography, increased ESI-MS sensitivity, easier sample preparation, high efficiency and long column lifetimes.
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TargetLynx
Waters
The TargetLynx™ Application Manager automates sample data acquisition, processing and reporting for quantitative results.It incorporates a range of confirmatory checks that identify samples that fall outside user-specified or regulatory thresholds. Identify Non-compliant Samples TargetLynx rapidly identifies and flags samples where: Analytes are above a specified maximum reporting level (MRL) Analyte confirmatory ion ratios are outside specified limits One or more analyte signal-to-noise ratios are below a defined value An analyte retention time or relative retention time is outside limits An analyte concentration is below set LOD and LOQ thresholds The standard deviation of response for QC standards exceeds a defined value The blank response is too high The coefficient of determination (r2) of the calibration curve exceeds a defined value LC/MS & LC/MS/MS and GC/MS & GC/MS/MS Use TargetLynx to quantify and confirm both LC/MS & LC/MS/MS and GC/MS & GC/MS/MS data. This data may be selected ion monitoring (SIM), multiple reaction monitoring (MRM), or full scan/full spectrum. In the case of full scan/spectrum data, extracted ion chromatograms are used for quantification. Ensure Regulatory Compliance The demand by regulatory authorities for assurance of confirmation in quantitative analysis continues to increase. To ensure cost-effective compliance, TargetLynx offers software that gives you confidence in your results and eliminates time-consuming, costly manual checks.
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Xevo TQ-S
Waters
Xevo TQ-S tandem quadrupole mass spectrometer - designed for the most demanding quantitative UPLC/MS/MS applications. The ultimate in tandem quadrupole performance allows you to achieve unrivalled sensitivity and robustness, available to everyone due to the accessibility of the Xevo platform. The Xevo TQ-S gives you class leading sensitivity and robustness, qualitative information when you need it most and accelerated method development for sensitive and robust methods. Reducing complexity, increasing ease of use, and ensuring the correct result every time.
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UHPLC and HPLCLC-MSData AnalysisChem / BioinformaticsCancer ResearchLipidsHuman PlasmaBiomarker Discovery

ACQUITY UPLC System

Waters

Waters ACQUITY UPLC - Dramatic Improvements in Speed, Sensitivity and Resolution Whether you work in methods development or quality assurance, in drug discovery and development or testing for safety in food supplies, you seek a productivity edge. With the ACQUITY UPLC® System, now you can get more information in a single, short run than you've ever seen with HPLC. Waters ACQUITY UPLC System UltraPerformance LC® (UPLC®) technology starts with unique 1.7 μm small-particle chemistries. Chromatographers no longer need to choose between speed and resolution–with UPLC you get both. Instrument Designed for Chemistry; Chemistry Designed for the Instrument The ACQUITY UPLC System has been holistically designed to match the performance needs of our innovative column chemistries with robust hardware, easy-to-use software and specialized support services. Small, pressure-tolerant particles High-pressure fluidic modules Minimized system volume Negligible carryover Reduced cycle times Fast response detectors Integrated system software and diagnostics With UPLC you have the ability to work more efficiently with higher speed, sensitivity and resolution at a much wider range of linear velocities, flow rates and backpressures to obtain superior results. The Waters ACQUITY UPLC System's high-pressure fluidics optimizes flow rates to make the most of small particle technology. The ACQUITY UPLC System's sample-handling design is designed to ensure exceptionally low carryover and reduced cycle time. And when interfaced with the Sample Organizer, it increases unattended sample capacity by ten times. High-speed detectors, both optical and mass, contribute to increased sensitivity and help manage the heightened speed and resolution requirements of UPLC. ACQUITY UPLC Systems are easily controlled, diagnosed, and monitored via a graphical system console interface. The console offers: Quick and easy access to critical instrument parameters Simple system start-up, elegant system status monitoring and predictive performance indicators to ensure maximum productivity Data management capabilities that are supported by both MassLynx™ and Empower™ software The ACQUITY UPLC System is also supported by Intelligent Device Management technology with our Connections® INSIGHT™ service, providing instrument diagnostics.

(167)

A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-Line Enrichment and Class Specific Separation of Phospholipids

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ACQUITY UPLC System

ACQUITY UPLC® BEH HILIC Columns

TargetLynx

Xevo TQ-S

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