Cell-Based Assays > Protein-Protein Interaction Assays Products & Reviews

High-throughput sialylation screening for expediting the clone selection process

The Mammalian Two-Hybrid Assay Kit is a powerful method for detecting protein-protein interactions in vivo in mammalian cells. The two-hybrid assay kit utilizes hybrid genes to detect protein-protein interactions via the activation of reporter-gene expression.  Detects protein-protein interactions in mammalian cells  Works well in a variety of mammalian cell lines  Confirms suspected interactions between two proteins or i…

NanoLuc® Binary Technology (NanoBiT) is a two-subunit system based on NanoLuc® luciferase that can be applied to the intracellular detection of protein:protein interactions (PPIs) in live cells. The NanoBiT® system is composed of two small subunits, Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide), that are expressed as fusions to target proteins of interest. The LgBiT and SmBiT subunits have been independ…

The NanoBRET™ Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. This target engagement assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells

BRET-Based Assays to Detect Protein:Protein Interactions in Live Cells

We offer extensive Antibody Functional Characterization Services, focusing on both Fab-based target binding and Fc-mediated effector functions. Our assays analyze mechanisms like Neutralization, ADCC, CDC, and ADCP, leveraging advanced platforms to select promising therapeutic candidates.

A service to identify the RNA targets of RNA-binding proteins (RBPs) on a genome-wide scale, providing insights into post-transcriptional regulatory networks.

Label-free, real-time analysis of biomolecular interactions to determine binding kinetics (kon, koff), affinity (KD), and specificity with high precision and sensitivity.

A suite of analytical services, including SPR, BLI, and ITC, to quantitatively characterize the binding kinetics, affinity, and thermodynamics of biomolecular interactions.

An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…

Absolute characterization of protein-protein and other macromolecular interactions with composition-gradient multi-angle light scattering.

This guideline describes the design, development, and validation of quantitative assays for proteins and peptides by mass spectrometry.

Quantify and characterize any protein interaction

Recombinant antigens and lentivirus for emerging SARS-CoV-2 variants