Protein Crystallography > Protein Crystallisation Reagents Products & Reviews

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.   The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in addition…

ProteaseMax™ Surfactant is a high-quality reagent for use in protein and peptide sample preparation.   ProteaseMax™ Surfactant solubilizes proteins and ensures fast and improved protein digestion with trypsin or chymotrypsin. It is designed to degrade over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). For…

The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.A highly effective…

Originally published in 1991 by Jancarik & Kim from conditions found to be successful in the crystallization of biological macromolecules. This classic standard sparse matrix screen lets you: Determine initial crystallization conditions. Establish the solubility of a macromolecule in a varying range of pH and precipitants. and with the enhanced buffer selection enables screening of greater crystallization space.Structure Scre…

The PCT Pre-Crystallization Test is used to determine the appropriate protein concentration for crystallization screening. Sample concentration is a significant crystallization variable. Samples too concentrated can result in amorphous precipitate, while samples too dilute can result in clear drops. Precipitate and clear drops are typical crystallization screen results for reagent conditions which do not promote crystallizati…

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules. The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a p…

SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH.SaltRx can be used as a primary crystallization screen when salt, ionic strength an…

Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format. Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in…

Individual reagents from the Grid Screen Ammonium Sulfate and Grid Screen Salt HT screens. Grid Screen Ammonium Sulfate Individual Reagents Features: 185 ml volume Sterile filtered Applications: Individual Grid Screen Ammonium Sulfate and Grid Screen Salt HT screen reagents for screening, optimization and production

Grid Screen Salt HT combines Grid Screen Ammonium Sulfate, Grid Screen Sodium Malonate, Quik Screen and Grid Screen Sodium Chloride into a single 96 Deep Well block format. The block contains 1 ml of each reagent. Features: A systematic grid screen varying salt or polymer or organic versus pH Samples pH 4 to 9 Combine reagents within or between screens to customize your screen Combine reagents between rows or colum…

The Steptavidin is produced by fermentation of S. avidinii in a minimal medium. The protein has a structure with four identical subunits, each containing a binding site for biotin. It is stable over a wide range of pH and decomposes only in the presence of SDS at temperatures above 60 °C. Steptavidin conjugated with an enzyme, i.e. Horseradish Peroxidase or Alkaline Phosphatase or a fluorochrome may be used to visualize biot…

The HRP is purified by repeated affinity chromatography of root extracts from horseradish. The result is an enzyme of high specific activity. Our horseradish peroxidase is purified in large scale by the use of benzhydroxamic acid agarose giving a product of high purity and specific activity. The product consists mainly of the basic isoenzymes and are supplied as lyophilized powder. Activity: >250 U/mg (25°C, Guaiacol as hydr…