Index Crystallization Screen

Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format.

Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in producing crystals or limiting sample solubility.

Results from Index can be used to design optimization experiments and to identify follow on screens by reagent class. For example, positive results with salt based reagent in Index may be followed up with further screening using SaltRx or Grid Screen Salt. Success with polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.


Index Crystallization Screen Features:

• A data-driven biased sparse matrix and grid screen
• Screens classic, contemporary, & modern crystallization reagents
• Samples pH 3 to 9
• Compatible with microbatch, vapor diffusion, & liquid diffusion methods
• Specially formulated reagent zones:
  - Traditional salts versus pH
  - Neutralized organic acids
  - High [salt] with low [polymer]
  - High [polymer] with low [salt]
  - Low ionic strength versus pH
  - PEG & Salt versus pH
  - PEG & Salt
• Tube or Deep Well block format

Applications:

• Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules