Gene Expression and Molecular Cloning Products & Reviews

A best-in-class DNA transfection reagent for your research.

Morphology driven high plex profiling on a single tissue section

The CosMx Spatial Molecular Imager is an FFPE-compatible, single-cell imaging solution that enables researchers to investigate intact tissues through the power of spatial multiomics.

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

The gold standard in bead-based NGS cleanup and size selection

NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, a…

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C ) Fast - 5 minutes for cohesive or blunt ends. Convenient - ligation performed at room temperature. Flexible - suitable for all common ligation reactions.

Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.

Twist Bioscience Gene Fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.

Unleash the full power of a well-designed library. With Twist Bioscience, you are assured that the library you design contains the specific modifications, exactly where you want them, saving you valuable resources for the downstream screening process.

Double-stranded DNA fragments for high-throughput screening applications

The LightCycler® 96 Instrument is a real-time PCR instrument for rapid cycling up to 96 samples. Applications include absolute and relative quantification, melting curve analysis, and endpoint genotyping. Precise LightCycler® 96 Instrument thermal homogeneity and cycling speed produce accurate and reproducible results in a very short time. The LightCycler® 96 Instrument’s optical detection system flexibly detects sequenc…

Human iPSC-derived glutamatergic neurons expressing Cas9 for rapid gene knockout generation

The Model P-97 micropipette puller is the latest generation of the Flaming/Brown type puller for fabrication of micropipettes, patch pipettes and microinjection needles. While retaining many of the features on the Model P-87, the P-97 offers improvements in mechanical, electronic and software design. The result is better control of the pulling process and a higher degree of reproducibility. Features: Environmental chamber…

FuGENE® HD Transfection Reagent is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in che…

Introducing the versatile reagent with the power to transfect your most difficult cells.

Contains one vial of Lipofectamine® 3000 Reagent (1.5 mL) and one vial of P3000™ Enhancer Reagent.

Ready-to-use, easy interpretation of results, Highly-sensitive, no need for internal control

SEQuoia Express Stranded RNA Library Prep Kit enables customers to overcome the challenge of a laborious library prep workflow by providing a cost-effective 3-hour, 3-tube, automation-friendly solution for constructing robust libraries that efficiently capture long RNA transcripts (>200bp).

Completely hands-off, walk-away HCP analysis in 1 hour.

The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by normalizing reporter expression to a control Optimized for SpectraMax® i3x, iD3, or L microplate readers with injectors…

High-throughput sialylation screening for expediting the clone selection process

Create your own custom microarray using eArray, Agilent's online design application, and focus on the targets of interest for your specific research needs.

The Agilent SurePrint G3 Human Exon Microarrays include probes targeting individual exons, making it possible to look beyond the gene to more subtle but relevant expression changes at the exon level. Agilent Exon Microarrays are the fast, simple, and cost-effective choice for exon expression analysis, with capabilities to drill down into important RNA changes at the exon level.