NEBuilder® HiFi DNA Assembly Cloning Kit
NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, a…
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Great for DNA assemblies
Cloning
I routinely use this for all DNA assemblies. At this point in my career, I have had other cloning methods fail too many times to bother using anything other than NEB Hifi. It works. It is robust.
Review Date: 14 Dec 2023 | New England Biolabs Inc.
Stop spending weeks on cloning.
Cloning
After learning cloning with standard restriction/ligation, I was introduced to Gibson assembly at the start of my postdoc. Using this, I've been able to clone large genes quite efficiently with up to 5 pieces to assemble. I would definitely recommend it to anyone. NEBuilder also has online tools to design your cloning and determine the amount of each part needed so tip top!
Review Date: 13 Jan 2023 | New England Biolabs Inc.
NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format.
- Assembly and transformation in just under two hours
- Flexible sequence design (scar-less cloning)
- No PCR clean-up step required
- High transformation efficiencies for inserts up to 20 kb
- Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
- Includes competent cells
