Collagenase
Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types 1, 2, 3, and 4 or chromatographically purified collagenase (the latter usually combined with secondary enzymes such as elastase, hyaluronidase, etc). For best results, the precise mixture of proteolytic activities must be tailored to the tissue to…
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Collagenase is dependable, and available by lot
Digest tissues in flow cytometry preparation
I appreciate Worthington's commitment to lot sampling and their focus on these tissue dissociation products. We use their collagenase II for digesting adult mouse myocardial tissue.
Review Date: 28 Feb 2023 | Worthington Biochemical Corp.
Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types 1, 2, 3, and 4 or chromatographically purified collagenase (the latter usually combined with secondary enzymes such as elastase, hyaluronidase, etc). For best results, the precise mixture of proteolytic activities must be tailored to the tissue to be dissociated. Correlations between type and effectiveness with different tissues have been good, but not perfect, and may be dependent partly on parameters of use and objectives as well as lot-to-lot variations.
Type 1: Crude collagenase has the original balance of collagenase, caseinase, clostripain and trypic activities
Type 2: Contains higher relative levels of protease activity, particularly clostripain
Type 3: Contains lowest levels of secondary proteases
Type 4: Designed to be especially low in tryptic activity to limit damage to membrane proteins and receptors
