PCR and Thermal Cycling Products & Reviews

Primer-only premixed assays for analyzing gene expression using intercalating dyes

RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara® Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.

Clara® HRM Mix delivers maximum specificity to your high resolution melt (HRM) curve analyses. Accurately detect genetic mutations, quickly identify genotypes based on SNPs, or calculate percent methylation of a target region with HRM analysis.

For nanoplate-based digital PCR applications

Accumax range of PCR plates & seals are the outcome of our impeccable standards of quality established throughout the manufacturing process resulting in the plates & seals that gaurantee precise and error free results every time.

QIAseq FastSelect: Increase your unique reads, discover more biology

QIAseq FastSelect: Increase your unique reads, discover more biology

The Applied Biosystems TaqPath Respiratory Viral Select Panel is a multiplex, real-time PCR (qPCR) in vitro diagnostic (IVD) test for identifying five common respiratory viruses in under three hours. The CE-IVD panel includes targets for adenovirus, human metapneumovirus, rhinovirus/enterovirus,* and parainfluenza** virus. 

The  therascreen  KRAS RGQ PCR Kit is a real-time, qualitative in vitro diagnostic test for the detection of 7 somatic mutations in the  KRAS  oncogene using a sample of DNA extracted from formalin-fixed, paraffin-embedded (FPPE) non-small cell lung cancer (NSCLC) tissue and colorectal cancer (CRC) tissue.

The InviScreen ® Norovirus Detection Kit provides a real-time RT-PCR method for the qualitative detection of norovirus genogroups I (GI) and II (GII) in food samples. The method is based on ISO 15216 and detects specific regions in the conserved 5′ end of the ORF1-ORF2 junction. The method is applicable to test samples of foodstuffs and surface swabs. The kit includes Infectious Bronchitis Virus (IBV) as a process control vi…

The InviScreen ® STEC Detection Kit provides a real-time PCR method for the qualitative detection of STEC E. coli in food samples. The method is based on ISO 13136 and detects the presence of the stx1 and stx2 genes (associated with cytotoxicity) and the eae gene which is associated with the capability of the bacteria to adhere to the intestinal epithelium and cause disease. The method is applicable to products intended for h…

The InviScreen ® Clostridium Botulinum Detection Kit is specifically designed for the qualitative detection of the specific botulinum neurotoxins (BoNTs) A, B, E and F of Clostridium botulinum in food, feed, and environmental samples. The real-time PCR procedure for detecting the presence of genes encoding BoNT type A (BoNT/A), type B (BoNT/B), type E (BoNT/E) and type F (BoNT/F) complies with the ISO/TS 17919 standard. The…

The InviScreen ® GMO Detection is a molecular biology tool that allows to easily detect genetically modified organisms (GMOs) using a fast, ISO 21569 standard-based, real-time PCR detection method based on the detection of the pCaMV35S, tNOS and pFMV genes. The kits can be used with a variety of starting materials, and benefits from the InviSorb® extraction kits that offer efficient protocols for DNA purification.

The InviScreen ® Allergen Detection is a premium quality molecular biology tool to provide the general laboratory and research personnel an easy and ready-to-use kit for the detection of specific allergen species in food and feed samples. For DNA purification prior to testing, the Invisorb ® extraction kits offer efficient protocols for various starting materials. Benefit from our real-time PCR detection technology and get ac…

VeriFi® Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.

96-well, thermal cycler for PCR with cables; for research use only.

Lentiviruses are commonly used as viral vectors in cell and gene therapy production, delivering and steadily integrating desired genes into the genome of the host cell. However, they may also present health hazards to humans due to integration-mediated transformation and the creation of a RCL that can infect nontarget cells. While the current regulatory guidelines recommend a cell culture–based method for RCL testing, it is cu…

Adeno-associated virus (AAV) is commonly used as a viral vector in cell and gene therapy production, but there is a risk of it becoming a replication competent virus (RCV) during the production process, especially in the presence of a helper virus, which can raise safety concerns. While the guidelines recommend a cell culture–based method for RCAAV testing, it is cumbersome and takes 30–45 days from sample to results. Verichec…

ZeptoMetrix® NATtrol™ is the trusted third-party QC for critical infectious disease testing. Ready-to-use, non-infectious whole microorganisms support full process monitoring, assay validation, and laboratory performance evaluation across molecular platforms.

Thermo Scientific Red Hot Taq DNA Polymerase consists of ThermoPrime Taq DNA Polymerase with an inert red dye to facilitate accurate low volume pipetting and as an indicator of enzyme addition. This dye has no adverse effect on the outcome of PCR; yields are the same as with standard ThermoPrime Taq DNA Polymerase. Highlights • Red colouring facilitates low volume pipetting • Reaction mix becomes pink on enzyme addition for ea…

Tube strips consist of eight 0.2 mL tubes connected together Cap strips are sold separately Made of thin wall polypropylene and designed for precise fit in heat blocks for optimum heat transfer Certified DNase- and RNase-free Autoclavable at 121°C

Thermo Fisher Scientific offers all ABgene PCR Master Mixes in a convenient pre-aliquoted format. This time-saving solution allows for more reproducible results by minimizing reagent handling steps and pipetting errors. Our Pre-aliquoted Master Mixes can be tailored to your very needs as custom primers can be added on request, and you can choose a plate or tube format that will suit your experimental set-up. Pre-aliquoted PCR…

This ready-to-use nucleotide mix is a premixed solution of multiple PCR-grade nucleotide sodium salts in water: dATP, dGTP, dCTP, and dTTP (each at a concentration of 10 mM). This mix is optimized for use in all types of amplification reactions and primer-extension reactions. PCR RT-PCR cDNA synthesis Primer extension Benefits Improve yield and performance Choose a convenient ready-to-use mix of all 4 nucleoti…

The Deoxynucleoside Triphosphate Set contains 4 individual vials of dATP, dCTP, dGTP, and dTTP, each as a 100 mM solution of the Na-salt. PCR-grade nucleotides are optimized for use in all types of amplification reactions and primer-extension reactions. Product Description PCR-Grade Nucleotides from Roche Applied Science are specially manufactured and purified to the highest possible chemical purity. PCR-Grade Nucleoti…

The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications: Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time instruments. Generating cDN…