MegaMix Gold RT-PCR Mastermix
The MegaMix Gold One-step RT-PCR kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit ensures a highly specific and sensitive amplification, removing background and primer dimer formation. MegaMix Gold boasts excellent accuracy and produces A-tailed products suitable for ligating into TA cloning vectors.
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MegaMix Gold One-step RT-PCR Kit contains all the components needed to perform RT-PCR swiftly and reliably.
The kit consists of 2X MegaMix Gold containing Hot Start Taq DNA polymerase, 200 μM dNTP and 3 mM MgCl2 (final concentrations) in Microzone’s proprietary enhancing buffer; 20X RTase/RI enzyme mix, optimised for amplifying low copy RNA targets; and Just Water, our molecular biology grade water. MegaMix Gold uses a superior sensitive hot-start DNA polymerase. The Taq polymerase becomes active upon heating at 95°C. This ensures a highly specific and sensitive amplification, removing background and primer dimer formation. MegaMix Gold boasts excellent accuracy and produces A-tailed products suitable for ligating into TA cloning vectors.
Key Features
- Hot Start polymerase in Microzone’s proprietary buffer gives unrivalled confidence in RT-PCR amplifications.
- 2X concentrated format.
- Broad range of templates and conditions.
- Extremely stable—can be freeze-thawed many times.
- Easy setup and RT-PCR optimisation.
Applications
- Hot Start RT-PCR up to 6 kb
- Amplification of any RNA template (mRNA, total RNA, viral RNA), low copy number genes
- RT-qPCR (probe or dye based)
- Endpoint RT-PCR
- Pathogen Detection
- Gene expression analysis.
- Amplification of GC and AT-rich templates
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