PCR and Thermal Cycling Products & Reviews

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Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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PCRBIO HiFi Polymerase

PCR Biosystems

PCRBIO HiFi Polymerase is our original high-fidelity enzyme, derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary point mutations allow for significantly improved performance when compared with its native form. Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR.

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PCRBIO Ultra Polymerase

PCR Biosystems

PCRBIO Ultra Polymerase has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

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PCRBIO Ultra Mix

PCR Biosystems

PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

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PCRBIO Ultra Mix Red

PCR Biosystems

PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates, with a red dye included for direct gel loading.

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UltraScript® cDNA Synthesis Kit

PCR Biosystems

UltraScript® cDNA Synthesis Kit is an easy-to-use 2-tube system specifically developed to generate cDNA for use in real-time PCR. The kit provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

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PCRBIO 1-Step Go RT-PCR Kit

PCR Biosystems

PCRBIO 1-Step Go RT-PCR Kit is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube. The advanced buffer system, reverse transcriptase and hot start polymerase give highly specific and ultra-sensitive 1-step RT-PCR from any RNA template including mRNA, total RNA and viral RNA sequences.

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Q-POC™ SARS-CoV-2

QuantuMDx

From collecting samples to reading results, the Q-POC™ SARS-CoV-2 assay has been developed considering patient and user experience at every step of the COVID-19 testing process. The test delivers rapid PCR results in approximately 30 minutes at the Point of Need, on the accurate and portable Q-POC™ platform.

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Q-POC™ SARS-CoV-2, Flu A/B & RSV Assay

QuantuMDx

Diagnosing, treating and managing clinically similar respiratory infections can be challenging.  The Q-POC™ SARS-CoV-2, Flu A/B & RSV multiplex assay provides rapid detection and differentiation of three leading respiratory diseases in just ~35 minutes. Running on the “one sample, one test” portable Q-POC™ platform, the test provides results you can trust at the Point of Need.

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  • Best New Clinical Assay or Kit of the Year



HiDi Taq 2x PCR Master Mix

Medix Biochemica

Highly discriminatative master mix for mismatches at the 3’-terminus of primers in PCR.  HiDi® Taq 2x PCR Master Mix is a ready-to-use aptamer-based fast-start formulated master mix specially optimized for assays in which high discrimination is required. With this unique mix less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.  

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Volcano3G RT-PCR Probe 2x Master Mix

Medix Biochemica

Volcano3G ® RT-PCR Probe 2x Master Mix contains an engineered, highly thermostable DNA Taq polymerase with reverse transcriptase activity that enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification.  

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PlexTaq 5x qPCR Multiplex Master Mix

Medix Biochemica

The 5x concentration of PlexTaq® 5x qPCR Multiplex Master Mix makes it optimal for multiplexing applications, leaving more free volume for primers and probes, and samples. The combination of aptamer-based fast-start Taq DNA polymerase, optimized reaction buffer, and ultrapure dNTPs, ensures consistent results, rapid set-up time, and a lower risk of pipetting errors.   

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MedixMDx qRT-PCR lyo ready mix

Medix Biochemica

 MedixMDx qRT-PCR Lyo ready mix is ready to be lyophilized to produce stable reagents at room temperature. Upon addition of target specific primers/probes and reverse transcriptase (RTase Lyo) to the mastermix, the mixture can be lyophilized directly, without the need to add additional excipients.  

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MedixMDx HiPlex qRT-Probe Mix No-ROX

Medix Biochemica

MedixMDx HiPlex qRT-Probe Mix is formulated as a 4x mix, which enables extensive multiplexing and addition of larger volumes of RNA templates to reactions. It includes an efficient thermostable reverse transcriptase with RNase inhibitor to prevent degradation of RNA templates by RNases.  

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PCR Tube

MEGAROBO Technologies

The PCR tubes are made of virgin medical grade PP with high chemical stability andultra-thin walls for extremely fast heat transfer and ramp-up time; thetubes are transparent for easy sample observation. 8-strip PCR tube adapted for rapid endpoint PCR and real-time fluorescent PCR.

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PCR Adhesive Seal

MEGAROBO Technologies

The PCR plates are made of virgin medical grade PP with high chemical stability and ultra-thin walls for extremely fast heat transfer and ramp up time. The plate com in non-skirt, half-skirt, and full skirted formats. The tubes of the plate are transparent for easy sample observation or white for appropriate light refraction.

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PCR Plate

MEGAROBO Technologies

The PCR plates are made of virgin medical grade PP with high chemical stability and ultra-thin walls for extremely fast heat transfer and ramp up time. The plate com in non-skirt, half-skirt, and full skirted formats. The tubes of the plate are transparent for easy sample observation or white for appropriate light refraction.

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QX600 Droplet Digital PCR System

Bio-Rad

The QX600 Droplet Digital PCR System (ddPCR™) offers advanced six-color multiplexing and absolute quantification of nucleic acid while delivering the best-in-class performance you have come to expect from Bio-Rad’s ddPCR systems.

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