ELISA > ELISA Reagents and Buffers Products & Reviews

Animal-free passive blocking antibody

Animal-free active blocking antibody

Gyrolab huIgG Kit – Low Titer is a ready-to-use Gyrolab kit for determination of human monoclonal antibody (mAb) titers up to 20 µg/mL.

Suitable for use in blocking for immunoprecipitation, immunocytochemistry, Western blotting, and dot blots.

Suitable for use in blocking for immunoprecipitation, immunocytochemistry, Western blotting and dot blots.

Suitable for use in blocking for immunoprecipitation, immunocytochemistry, Western blotting and dot blots.

Suitable for use in blocking for immunoprecipitation, immunocytochemistry, Western blotting and dot blots.

Suitable for use in blocking for immunoprecipitation, immunocytochemistry, Western blotting and dot blots.

Agarose Dissolving Buffer is designed for use in the Zymoclean family of products. This buffer dissolves agarose gel slices for the recovery of DNA and facilitates efficient DNA binding and subsequent recovery.

Our unique combination of custom antibody development, lateral flow  development and manufacturing services, signal enhancement technology and our mobile diagnostic platform are shaping the future of lateral flow. Our partnership approach allows you to work with one company to reach your assay goals.

Discover our breadth of experience and be reassured that our Lateral Flow  Development Services will deliver the best outcome for your assay.

BBI Solutions has over 25 years’ expertise working with customers and partners to develop and manufacture high quality proteins for a diverse range of applications with our Custom Protein Service.

BBI’s custom conjugation service allows us to develop high-quality conjugates using reagents provided by you or sourced by us.

BBI Solutions’ Novarum™ technology transforms a smartphone into a mobile diagnostic platform, empowering users to read and share the results of rapid tests at the point of care.  

The Gyrolab® 2G Generic PK Kit enables high sensitivity quantitation of intact human IgG (IgG1, IgG2, IgG4 subtypes) in preclinical matrices

ApplicationThe kit is used for the quantitative determination of BrdU incorporated into cellular DNA using a 96-well microplate cell ELISA format. Benefits Safer: No radioisotopes are used. Accurate: The results strongly correlate to those obtained with the [3H]-thymidine method. Sensitive: As sensitive as [3H]-thymidine. Fast: The use of multiwell ELISA readers allows for processing of a large number of samples. Ea…

The Alkaline Phosphatase is purified from calf intestine by a method developed in our laboratories, involving affinity chromatography. This enzyme is widely used as a marker enzyme in applications such as: • ELISA Immunoblotting Immunohistochemistry Molecular Biology Activity: >630 U*/mg enzyme protein (25°C, 4-nitrophenyl phosphate, glycine as buffer) • Unit definition: That amount of enzyme causing the hydrolysis of one mi…

The protein-free Blocking Buffer is a ready-to-use solution intended for blocking of the remaining free binding sites after microplate coating. The blocking is highly effective and stable. Our Synthetic Blocking Buffer™ eliminates the risk of false positive cross reactions in the assay. Current safety hazards related to the use of bovine material, for instance BSA are avoided. The buffer is ready-to-use and offers a high level…

OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…

OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…

OPD is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. Stability life time is 18 months at 4°C. Our standard tablets are available with a content of 2 mg or 10 mg OPD.

DAB (3,3-diaminobenzidine tetrahydrochloride), is used as a chromogen substrate in immunoblotting.DAB produces a brown colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 10 mg of DAB and dissolves in distilled water in less than 5 minutes. Stability life time is 18 months at 4°C.

Fast Red is used as a chromogen substrate in immunoblotting and immunohistochemistry using alkaline phosphatase antibody conjugates. Each tablet contains 0.5 mg naphthol phosphate, 2 mg FAST RED chromogen and 0.4 mg levamisole, an inhibitor of endogenous enzyme activity. Stability life time is 18 months at 4°C.

The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add equal amounts of 1 .0 M s…

The liquid BCIP/NBT is the most commonly used substrate for colorimetric determination of Alkaline Phosphatase (AP) activity in membrane tests. We offer an aqueous buffered solution containing NBT (NitroBlue Tetrazolium) and BCIP (Bromo-Chloro-Indolyl Phosphate) forming a blue/purple precipitate at the positive sites. The colour develops when AP catalyses the dephosphorylation of BCIP. The intense blue/purple precipitate is…