ELISA > ELISA Reagents and Buffers Products & Reviews

SA10 Streptavidin (formerly ProZyme) is Agilent's longest-established streptavidin product, being a widely-used workhorse of the industry for over 25 years. It is purified to a high degree from fermentations of Streptomyces avidinii and has a molecular weight of 52 kDa (also known as 'core' streptavidin). Agilent also offer a variant of streptavidin (SA26 Streptavidin-plus), which is made as a recombinant form in E. coli an…

The AttoPhos® AP Fluorescent Substrate System provides a highly sensitive fluorescent alkaline phosphatase substrate.   The system includes AttoPhos® Substrate, AttoPhos® Buffer and Calibration Solution. AttoPhos® Substrate (2′-[2-benzothiazoyl]-6′-hydroxybenzothiazole phosphate [BBTP]) is cleaved by alkaline phosphatase to produce inorganic phosphate (Pi) and the alcohol, 2′-[2-benzothiazoyl]-6´-hydroxybenzothiazole (BB…

Stable Isotope-labeled and Unlabeled Buffers, Reagents, Lipids, and Detergents

The Luminata ELISA HRP chemiluminescent substrates provide three major advantages:High sensitivity for detecting as little as 0.1 pg of proteinBroad dynamic detection range (up to 2,500-fold), enabling a wide range of quantitation.Flexibility to adjust ELISA signal strength

Neptune™ HRP Conjugate Stabilizer preserves the activity of HRP-protein conjugates. This HRP stabilizer can be used to store concentrated stock HRP conjugates, reconstitute lyophilized conjugates, and dilute HRP conjugated antibodies to their useful working titer in ELISA tests and immunology-based techniques.Neptune™ HRP Conjugate Stabilizer is especially suited for ELISAs using anti-IgG HRP conjugates within traditional ant…

General Block contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide or protein antigen-down ELISA formats. General Block contains a mammalian protein blocking agent designed to reduce nonspecific background noise in most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. This unique, low-level blocking buffer minimizes nonspecific binding interacti…

Neptune Block Blocking Buffer is designed primarily for antigen-down ELISA formats as well as sandwich assays with high background problems. Neptune Block ELISA Blocking Buffer is particularly useful when working with human and other mammalian serum samples as it works to reduce interactions between sample and blocking molecules.Neptune Block ELISA Blocking Buffer is a mixture of small molecular proteins and molecular stabili…

Eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. SynBlock ELISA Blocking Buffer provides superior background-blocking performance without the use of conventional cross-reactive protein additives. SynBlock ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with…

Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs.Phosph-Free Block is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. However, it is appropriate for assays using HRP detection as well. Using inert no…

Reduce non specific binding in ELISAs and stabilize proteins with Alternative Block, an all-synthetic ELISA blocking buffer free of proteins and detergents. Alternative Block is a protein-free, detergent-free ELISA blocking buffer. This unique blocking buffer contains a heterogeneous mixture of proprietary blockers and synthetic stabilizers that block the uncoated regions of the microtiter plate without the use of conventiona…

Reduce non specific binding in ELISAs, address high background issues, and stabilize proteins with Monster Block, a new ELISA blocking buffer utilizing a heterogenous mixture of nonmammalian blocking agents. Monster Block™ provides a high degree of blocking efficiency and protein stabilization through the use of non-mammalian protein blocking agents and stabilizers. The heterogeneous composition of this unique ELISA blocking…

Peroxidase labelling of proteins or amine compounds.  Only 3 hours to get conjugates, all processes in a single filtration tube, high recovery of conjugates.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Serpin E1 / PAI-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

DRG International can offer a wide range of sensitive and reliable ELISAs for measurement of free steroid hormones as well as other parameters in saliva. Recently DRG has developed a new and unique ELISA for the determation of  Hepcidin in Saliva.  Determination of Hepcidin-25 in saliva has the advantage of a non-invasive and stress-free sample collection without the need of medical personnel.

2 ml, EIA-grade, enzyme-antibody conjugate

1 ml, EIA-grade, enzyme-antibody conjugate

1 ml, EIA-grade, enzyme-antibody conjugate

1 ml, EIA-grade, enzyme-antibody conjugate

1 ml, EIA-grade, enzyme-antibody conjugate

1 ml, EIA-grade, enzyme-antibody conjugate

Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml

Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm

100 ml, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm

1 L, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm

Antibody isotyping kit for ELISA, sufficient for 100 typings (800 tests), includes mouse isotyping panel (#172-2055), affinity purified goat anti-rabbit IgG (H + L)