Gene Expression and Molecular Cloning Products & Reviews

Achieve ultra-high RNA yields with MEGAscript™ kits—ideal for in vitro or in vivo translation studies needing unlabeled or low specific activity RNA.

Remove cap structures (decapping) from various RNAs. Substitute unit for unit into any prexisting protocol for Tobacco Acid Pyrophosphatase (TAP).

Build Cap 0 or Cap 1 caps with nearly 100% efficiency. Provides all reagents for capping in vitro transcribed RNA.

Prepare Cap 1-RNA from any source of Cap 0-RNA. Use simultaneously with ScriptCap™ m7G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction.

Generate pseudouridine-containing mRNA (Ψ-mRNA) with greatly reduced double-stranded RNA (dsRNA) content. Easily remove dsRNA without the need for chromatographic methods like HPLC. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells.

Specially formulated for maximum yields of canonical (GAUC) in vitro transcribed RNA. Produce up to 180 µg of RNA from 1 µg of template in just 30 minutes. Easily scale up reactions to produce milligram amounts of RNA.

Optimized for high-yield synthesis of 5-methyl-cytidine- pseudouridine-containing, in vitro transcribed RNA (5mCΨ-RNA). Produce up to 100 µg of 5mCΨ-RNA from 1 µg of template in 2 hours. Easily scale up to produce milligram amounts of 5mCΨ-RNA. Induce lower innate immune responses in mammalian cells by using 5mCΨ-mRNAs.

Quantitative mRNA poly(A) tail length using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis. Assess tail lengths above the limits of HPLC and LC-MS based methods.

Quantitate mRNA capping efficiency (percent capped RNA content) of XBG 5’ UTR-containing mRNA using standard polyacrylamide gel electrophoresis (PAGE). XBG 5’ UTR Targeting Oligonucleotide and XBG 5’ UTR Control Mix included in kit. Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis.

Easy to use, scalable enzymatic method for removing double-stranded RNA (dsRNA) from RNA samples. Remove dsRNA without the need for chromatographic methods like HPLC. No reduction in single-stranded RNA (ssRNA) yields. Reduces the innate immunogenic response to mRNA in cells.

Amplify a CRISPR guide sequence derived from a CROP-style vector alongside your standard RNA Sequencing Library.

Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction.

Optimized for high-yield synthesis of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 100 µg of Ψ-RNA from 1 µg of template in 2 hours. Induce lower innate immune responses in mammalian cells by using Ψ-mRNA.

All-in-one kit to produce N1-methyl-pseudouridine-containing-mRNA (N1meΨ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of N1meΨ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using N1meΨ-mRNAs.

Digests linear RNA and Y-structure RNA, leaving circRNA, lariat intron RNA, and dsRNA intact. Ideal for isolating circRNA from total RNA preparations. 1 µg of RNase R digests 5 µg of linear RNA in 30 minutes.

Human iPSC-derived microglia expressing Cas9 for rapid gene knockout generation

UltraScript® cDNA Synthesis Kit Separate Oligos contains all the components for efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Anchored oligo(dT) and random hexamers are provided separately for user optimisation depending on the type of analysis needed.

Specially formulated for maximum yields of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 160 µg of Ψ-RNA from 1 µg of template in 1 hour. Easily scale up to produce milligram amounts of Ψ-RNA. Induce lower innate immune responses in mammalian cells by using Ψ-mRNAs.

UltraScript 2.0 cDNA Synthesis Kit Separate Oligos containins all the components required for fast, reliable and unbiased cDNA synthesis of the most challenging RNA sample types. Anchored oligo(dT) and random hexamers are provided separately for user optimisation depending on the type of analysis needed.

Optimized for high-yield synthesis of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 60 µg of Ψ-RNA from 1 µg of template in 2 hours. Easily scale up to produce milligram amounts of Ψ-RNA. Induce lower innate immune responses in mammalian cells by using Ψ-mRNA.

For post-transcriptional poly(A) tail addition to RNAs. Tail mRNAs in vitro to lengths equivalent to or greater than those found in nature.

Co-transcriptionally cap 5-methyl-cytidine- pseudouridine-containing, in vitro transcribed RNA with Anti-Reverse cap analog (ARCA). Produce up to 40 µg of capped 5mCΨ-RNA from 1 µg of template in 3 hours. Optimized for maximum transcript capping and yield. Induce lower innate immune responses in mammalian cells by using 5mCΨ-mRNAs.

Quantitate mRNA capping efficiency (percent capped RNA content) using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis.

Quantitate the percentage of mRNA with Cap 1 vs Cap 0 5’-cap structures using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis.

All-in-one kit to produce pseudouridine-containing-mRNA (Ψ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using Ψ-mRNAs.