Oligo Pools
Twist Oligo Pools are highly diverse collections of single-stranded oligonucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high-quality, accurate oligos per run.

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Oligo pools are utilized in many applications including generation of CRISPR guide RNA (sgRNA) libraries, peptide screens, protein screens, and high-throughput reporter assays. Accuracy and uniformity of oligo synthesis are critical to ensure high quality oligo libraries are built and screens are precisely targeted. Our industry-leading low synthesis error rate and uniform sequence representation result in high quality custom oligo libraries that enable the most efficient screens.
SPECS:
- Oligo Length: Up to 300 nt
- Oligo Pool Size: Minimum of 2,000 sequences with no maximum
- Yield>0.2 fmol for each oligo













