PCR and Thermal Cycling Products & Reviews

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so tha…

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Mast…

Original Eppendorf PCR tubes are manufactured to the highest Eppendorf quality standards. These thin-walled polypropylene tubes ensure efficient and homogeneous heat transfer in the sample thanks to the even wall thickness and smooth wall surface. Heat-sealed bags enclosed in boxes sealed with a PCR Clean sticker ensure the highest degree of purity. A frosted labeling area on the tube allows easy sample tracking

Product features Pink or light blue above 7°C, violet or dark blue when ice-cold DescriptionThe new Eppendorf PCR-Cooler maintains a sample temperature of 0°C*1 for over an hour and then changes color when the temperature has exceeded 7°C. Superior to an ice bath, PCR-Cooler is easy to handle, space-saving and free of contamination, which makes it the perfect preparation for your PCR reactions. The temperature of single tub…

Original Eppendorf PCR tubes are manufactured to the highest Eppendorf quality standards. These thin-walled polypropylene tubes ensure efficient and homogeneous heat transfer in the sample thanks to the even wall thickness and smooth wall surface. Heat-sealed bags enclosed in boxes sealed with a PCR Clean sticker ensure the highest degree of purity.

These automated pipetting systems are perfectly suited for PCR and qPCR setup, nucleic acid purification as well as low-throughput NGS library preparation yet they retain the flexibility for use as open platforms for diverse automated liquid handling applications. The epMotion 5073 series automates and simplifies traditionally complex, labor-intensive pipetting tasks, saving time and improving the reliability and reproducibili…

The DNA LoBind Tube finally provides the genomics user with the certainty that sample loss of genetic material caused by interaction with the plastic surface of the tube will be insignificant. With the combination of the purest selected types of polypropylene and specially developed production technologies, a tube has been developed that ensures nearly 100% recovery of DNA/RNA. A comparison of the recovery rates of various p…

The Mastercycler nexus X2 gives you the ability to run two totally independent protocols at the same time. Smaller assays fit nicely on the 32-well-block — larger assays can exceed 48 samples and run on the 64-well-block. The larger block is available with a gradient function. As every member of the Mastercycler nexus family, the Mastercycler nexus X2 can be combined with all other family members in a network of up to 3 units.…

The new Mastercycler® X50 elegantly combines speed, flexibility, and ease of use. With its intuitive touch screen offering precise fingertip control, the MC X50 PCR thermocycler enables easy PCR optimization in advanced molecular biology research and dependable standardization in routine PCR applications. It is also perfectly fitted for NGS.

Eppendorf real-time PCR Tube Strips feature an extremely thin wall for optimal heat transfer combined with high mechanical stability. In combination with their optical properties these are the ideal tube strips for any real-time PCR, especially for use with small reaction volumes. The Masterclear™ Cap Strips offer further advantages for your real-time PCR. The inverted dome of the Masterclear™ Cap Strips prevents scratching or…

Twist Bioscience, the leader in synthetic DNA with unparalleled precision at scale, is redefining targeted sequencing performance with the Twist Human Core Exome Kit. This kit includes library preparation and enrichment components, allowing the flexibility to lower sequencing costs, increase sample throughput, and achieve a higher depth of coverage across target regions with uncompromising quality.

Positive controls provide quality control measures for the verification and validation for point-of-care diagnostic tests including both next-generation sequencing (NGS) and reverse transcriptase-polymerase chain reaction (RT-PCR) assays. Twist offers fully synthetic SARS-CoV-2 RNA controls, available for dozens of distinct reference sequences.

Manufactured from medical grade polypropylene

Thermal cycler system, includes 96-well thermal cycler, power cord, tube support ring

Takara produce a host of products for PCR Takara’s new sensitive qPCR premixes contains TLi RNase H and are developed to address the inhibitory effect on the PCR due to the presence of remaining RNA after cDNA synthesis. PrimeSTAR®, a non-Taq polymerase with high proofreading activity are now available in a high speed version, PrimeSTAR Max, with extension rate of less than 5 sek/kb and PrimeSTAR GXL, that amplifies up to 30 k…

Fosmid clones are similar in size (ca. 40kb) to cosmids, but, like BAC clones, they contain replicons derived from the F factor for DNA replication and segregation. Because of this property, they are present in low copy number and consequently more stable than cosmids. Also, the copy control vector can be used for easy DNA purification from fosmid clones. Fosmid libraries are commonly used for closing gaps in a whole genome se…

The miScript miRNA PCR Arrays accurately analyze the expression of multiple microRNA (miRNA) sequences at the same time on ANY real-time PCR instrument. This new generation of miRNA PCR Array builds on the original strengths of the RT² miRNA PCR Arrays* with new pathway content, expanded assay coverage plus added species all with the flexibility and sample preparation advantages of the miScript PCR System. The available 96-…

The Applied Biosystems 7300 Real-Time PCR System gives you advanced, multicolor real-time PCR capabilities with exceptional budget sensitivity. Run time of less than 2 hours Four-color detection enables you to perform gene expression analysis, pathogen quantitation, SNP genotyping, and plus/minus assays with internal positive controls Study gene expression, detect and quantitate pathogens, perform allelic discrimination…

Robotic workstation for fully automated nucleic acid preparation and filling of LightCycler® Capillaries, 96-well PCR plates, and tubes suitable for the most commonly used PCR instruments Benefits Save time with rapid, fully automated isolation of high-quality DNA, RNA, and mRNA. Obtain reliable, reproducible results. Benefit from high flexibility in sample type and volume, as well as elution volume and post-elution p…

TaqMan® Gene Expression Assays are a comprehensive collection of over 1,000,000 probe and primer sets for quantitative gene expression analysis of 13 species including human, mouse, rat, Arabidopsis, Drosophila, and C. elegans genes. These assays let you quickly, easily, and affordably perform precise gene expression studies using real-time PCR. TaqMan®-based assays provide unmatched sensitivity, specificity, and reproducibi…

With over 4.5 million SNP assays available, including 3.5 million HapMap SNPs, 70K cSNPs, and 160,000 validated assays, Applied Biosystems SNP Genotyping Assays make it easy to perform SNP genotyping studies with the precision of TaqMan® reagent-based chemistry. These assays provide SNP detection capabilities with high-confidence results. The large selection of SNP assays helps advance your screening, association, candidate…

Applied Biosystems GeneAmp® Fast PCR Master Mix's sensitive, pre-mixed hot-start chemistries reduce PCR reaction time to as little as 25 minutes when used with the 9800 Fast PCR System. • Pre-mixed hot-start chemistry enables you to set up your experiments quickly. • Detect a single copy of a gene in as little as 10 ng of DNA. • Get reproducible, specific, and sensitive results.

Extensor Long Range Enzyme Blend is an optimized mix of ThermoPrime* DNA Polymerase and a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate long PCR products, up to 20kb. This makes the enzyme blend ideal for robust full-length amplification of long range sequences. ■ Amplifies targets up to 20kb in length ■ Unique enzyme blend and optimized buffer combination delivers increased accu…