Gene Expression and Molecular Cloning Products & Reviews

Reliable Transfection for Common Cell Lines

Capped or Uncapped RNA Transcripts for in vitro or in vivo Translation

MOA-based functional bioassay to measure Fc effector activity.

The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at l…

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample…

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize s…

The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products.   They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the…

Predesigned probe assays for gene expression analysis

Broad portfolio of products for knockdown of gene expression

Modulation of gene expression with modified oligos

FDA approved Modern cellular and molecular biology poses the strictest requirements for the usage of sensitive samples such as living cells, nucleic acids or other cell components. With this ambition of quality in mind, Eppendorf offers a large selection of microcapillaries for the most varied applications. VacuTips capillaries and TransferTips® for intracytoplasmatic sperm injection (ICSI ) TransferTip (ES) for embryo…

The new InjectMan® 4 is ideal for microinjection into adherent cells, smaller organisms and embryos in the early stages of development. The combination of InjectMan 4, FemtoJet® 4i or 4x enables a fast, semi-automatic injection. Furthermore, the InjectMan 4 is the ideal micromanipulator for all complex applications that require a dynamic movement mode and direct control of the injection process via the joystick button. The ax…

Type VI-S, lyophilized powder, for diagnostic assay manufacturing

Type I-S, 65-85% protein (≥75% of protein is clottable)

1,6-α-D-Glucan 6-glucanohydrolase

Recombinant, expressed in Pichia pastoris, ATPase ≥1000 units/mg protein, lyophilized powder.

Fast-track your route to a modernized workflow with the only in vitro bioavailability assessment tool on the market

Creative Biolabs delivers an end-to-end CAR-T development pathway—from target/scFv discovery, CAR design and vectoring, and cell engineering to in-vitro/in-vivo efficacy, IND authoring, and manufacturing support. Modular and GMP-aligned, the platform spans autologous and allogeneic routes (incl. TRAC/Cas9 and mRNA) to de-risk translation for hematologic and solid tumor programs.

End‑to‑end solid‑tumor CAR‑T development—from antigen/scFv discovery and CAR optimization to cell engineering, in‑vitro/in‑vivo efficacy, and IND support. Incorporates dual/logic‑gated and armored CARs, plus trafficking and persistence enhancements to overcome the tumor microenvironment and improve safety and translation.

End‑to‑end allogeneic cell therapy development—covering donor strategy, multiplex genome editing for safety/immune evasion, scalable manufacturing and comparability, and IND/CMC support—across CAR‑T/CAR‑NK and other donor‑derived modalities to enable off‑the‑shelf access.

Specially formulated for high yields of canonical (GAUC) in vitro transcribed RNA. Produce up to 90 µg of RNA from 1 µg of template in 2 hours. Easily scale up reactions to produce milligram amounts of RNA.

Amplify micrograms of circular DNA from as little as 5 pg of input. Directly amplify from purified plasmid, glycerol stocks, liquid media cultures, or colonies on agar plates. Accurate amplification, with an error rate of ~1 in 106-107 bases.

Build Cap 0 caps with nearly 100% efficiency. Use simultaneously with ScriptCap™ 2'-O-Methyltransferase to produce Cap 1 caps.

Co-transcriptionally cap in vitro transcribed RNA with Anti-Reverse cap analog (ARCA). Produce up to 60 µg of capped RNA from 1 µg of template in just 30 minutes. Optimized for maximum transcript capping and yield.