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pGEM®-T Easy Vector Systems

Promega Corp.A1360

The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products. They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the…

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Description

Description

The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products.

They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the pGEM(R)-T Easy Vector System I components.

pGEM®-T Easy Vector Systems Features & Benefits:

Flexibility - The multiple cloning site is flanked by restriction enzyme sites for BstZI, NotI and EcoRI, allowing three options to remove the insert with a single digest.
Rapid Ligation - The 2X Rapid Ligation Buffer provided allows reactions to be completed in 1 hour at room temperature.
Blue/White Screening - T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by color screening on indicator plates.
f1 Origin of Replication - Allows preparation of single-stranded DNA.

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pGEM®-T Easy Vector Systems - Promega Corp. - Life Sciences

pGEM®-T Easy Vector Systems

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