Life Sciences Products & Reviews

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.   Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits:   Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…

GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polyemrase in a specially formulated mixture with a proprietary thermostable proofreading polyemrase.   This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polyemrase allows the polyemras…

QuantiFluor® ONE system maximizes sensitivity and efficiency for dsDNA quantitation.   The QuantiFluor® ONE dsDNA System contains a fluorescent double-stranded DNA-binding dye (504nmEx/531nmEm) developed for use in an “add-and-read” format for dye and standard, making sample quantitation easy. This system enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA). The QuantiFluor® ONE dsDNA Sy…

This ready-to-use multimode detection system includes: high-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities.   The GloMax® Discover System is a high-performance multimode detection instrument developed with Promega reagent chemistries to provide a simple means of detecting advanced chemistries. This instrument provides…

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase.   The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary scre…

The Maxwell® Clinical Sample Concentrator (CSC) automated nucleic acid extraction system is designed for clinical laboratories needing to process multiple samples for critical downstream assays. This easy-to-use, cGMP-compliant system provides consistent, high-quality results with every run, allowing you to manage your workflow and gain efficiency. The purification methods use sample lysis and binding to paramagnetic par…

Designed for use with the Maxwell Clinical Sample Concentrator and the Maxwell CSC blood purification method as an IVD medical device for automated isolation of human genomic DNA from whole blood.   Because the Maxwell® CSC Blood DNA Kit is manufactured to such strict specifications, you can count on consistent, high quality DNA suitable for use in downstream amplification assays. Furthermore the intuitive interface…

The Maxwell® CSC DNA FFPE Kit is used in combination with the Maxwell® CSC Instrument to provide an easy method for efficient, automated purification of genomic DNA (gDNA) from human FFPE breast, lung or colon tissue samples.   The Maxwell® CSC DNA FFPE (formalin fixed, paraffin embedded) kit is intended for use, in combination with the Maxwell® CSC Instrument and the Maxwell® CSC DNA FFPE purification method, as an…

The Maxwell® 16 LEV simplyRNA Kits are for use with the Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor.   This RNA purification procedure is a simple method with minimal lysate handling before automated purification on the Maxwell® 16 Instrument. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such…

The QuantiFluor® ONE dsDNA Dye is a fluorescent double-stranded DNA-binding dye (504nmEx/531nmEm) prepared for an “add-and-read” format making sample quantitation easy. This dye enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA).   The QuantiFluor® ONE dsDNA Dye is intended for use as a general purpose reagent in the US and is suitable for use by clinical laboratories as a component of la…

The Maxwell® 16 LEV RNA FFPE Purification Kit is designed to extract RNA rapidly from FFPE samples using the Maxwell® 16 Instrument (Cat.# AS2000 and AS3000).   The FFPE kit is specifically designed for optimal purification of RNA from 5μm thin sections of formalin-fixed, paraffin-embedded (FFPE) tissue samples. The Maxwell® 16 LEV RNA FFPE Purification Kit is optimized to yield a final RNA concentration that maximi…

The CellTiter-Glo® 3D Cell Viability Assay is a homogeneous method to determine the number of viable cells in 3D cell culture based on quantitation of the ATP present, which is a marker for the presence of metabolically active cells.   This ready-to-use reagent is based on the original CellTiter-Glo® Luminescent Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when prepa…

The mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications.   The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spectrometry (LC/MS) analysis. Both the yeast and human extracts also are available in an intact undigested form to provide a test material for optimizing mass spec protei…

The GloMax® Explorer System is the newest member of the GloMax® System detection instruments. This multimode reader allows you to get up and running quickly, generating the data you need from your experiments. Simply unpack it, plug it in, and begin your experiments.

The PureYield™ Plasmid Maxiprep System isolates transfection-quality plasmid DNA. The system provides a rapid method for purification of up to 1mg of plasmid DNA from a 250ml bacterial culture. Plasmid DNA can be purified rapidly with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. The PureYield™ Plasmid Maxiprep System incorporates a unique End…

NanoLuc® Binary Technology (NanoBiT) is a two-subunit system based on NanoLuc® luciferase that can be applied to the intracellular detection of protein:protein interactions (PPIs) in live cells. The NanoBiT® system is composed of two small subunits, Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide), that are expressed as fusions to target proteins of interest. The LgBiT and SmBiT subunits have been independ…

To shorten the time required to prepare samples for LC-MS/MS analysis, we have developed an optimized protocol in which rapid and efficient trypsin digestion is achieved at temperatures as high as 70°C. Using this approach, proteolytis reaction times are dramatically shortened, no chemical denaturants are added and off-line sample cleanup is unnecessary, leading to shorter preparation times and diminished sample losses.

The Lactate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-lactate in biological samples. Lactate is produced by glycolysis, a major metabolic pathway responsible for glucose homeostasis and energy production (1,2). Once considered merely a byproduct of glycolysis, lactate is now considered an important regulatory molecule of intermediate metabolism involved in cancer development, diabet…

The Glucose-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glucose in biological samples.

The Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamate in biological samples.

The Glutamine/Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples.

The Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay is a bioluminescent assay for detecting the activity of JumonjiC (JmjC) histone demethylases and many Fe(II)/α-ketoglutarate-dependent dioxygenases that use α-ketoglutarate as substrate and release succinate as a product.

Count on minimal interference from test compounds when screening your compound library using luminescent, ATP-based kinase assays. We offer the ADP-Glo™ Kinase Assay, Kinase Selectivity Profiling Systems and hundreds of Kinase Enzyme Systems. These sensitive, reliable assays are easily scaled to meet your throughput needs and can be used with virtually any kinase and kinase substrate combination.

The NanoBRET™ Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. This target engagement assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It is an engineered recombinant protease overexpressed in E. coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS Protease to the IgG sample, add 1 unit of IdeS Protease per 1µg of IgG to be digested…