This column allows highly reproducible analyses of fatty acid methyl esters (FAMEs), specifically the omega 3 and omega 6 fatty acids. It is tested to ensure reproducible FAME equivalent chain length (ECL) values and resolution of key components. Column Details: Phase: Bonded; poly(ethylene glycol) USP Code: G16 Length: 15, 30 m ID: 0.10, 0.25, 0.32, 0.53 mm df: 0.10, 0.25, 0.50
C18 HPLC column for High Speed and High Resolution Applications. Ascentis® Express C18 is the first choice for starting a new method. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles at lower system pressure. With identical conditions, the Ascentis Express C18 column performs more than 4X faster tha…
Classic reversed-phases for all general-purpose applications. Features: Excellent peak shape and efficiency compared to competitive columns Classic reversed-phase retention and selectivity Excellent, no-bleed character for LC-MS applications C18 is generally more retentive than the C8 Column Details: Chemistry: C18 Particle Size: 5 µm Pore Size: 180 Å Length: 20, 30, 50, 100, 125, 150, 250 mm ID: 2.1, 3.0, 4.…
Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users. Column Details: Mode of chromatography: Hydrophilic interaction…
Application: The 35% phenyl equivalent phase provides a higher polarity option compared to columns containing a lower phenyl content, resulting in a greater retention of polar analytes relative to non-polar compounds. Its selectivity is complementary when paired with SLB-5ms for applications that require dual-column confirmatory analysis, such as for environmental analytes (such as pesticides, PCBs, and herbicides). The low…
The Next Generation in Hybridspe-Phospholipid Technology
The Ascentis Express biphenyl phase chemistry offers alternative selectivity for pharmaceutical analytes and drug metabolites that are not well retained or difficult to resolve on traditional alkyl (C18) and phenyl bonded phases. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 ?m particles while maintaining lower backp…
Sufficient for 100 colorimetric or fluorometric tests
BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid
2 μg/mL in acetonitrile, analytical standard
sufficient for 200 fluorometric tests
Certified reference material, 20 μg/mL in methanol, ampule of 1 mL
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein.
The polar organic and the polar ionic modes have become increasingly popular largely because of their ideal compatibility with MS detection and preparative LC. Two years of extensive research at Astec in understanding these mechanisms has resulted in new possibilities for extending their effectiveness even further. Important changes in linkage chemistries and the silica and the silica have now led to the new CHIROBIOTIC V2 and…
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we h…
Patent pending HybridSPE – Precipitation (HybridSPE-PPT) technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then…
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase,…
