Glucose and Sucrose Assay Kit
Sufficient for 100 colorimetric or fluorometric tests
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Sucrose is a disaccharide that is hydrolyzed to glucose and fructose by invertase. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Upon cellular uptake, glucose can be shunted towards breakdown via glycolysis or polymerized for energy storage.
In this assay kit, glucose is oxidized via glucose oxidase resulting in a colorimetric (570 nm)/ fluorometric (lex = 535 nm/lem = 587 nm) product, proportional to the glucose present. To measure sucrose, invertase is added to the reaction to convert the sucrose to glucose and fructose. The free glucose can be subtracted from the total glucose to give the concentration of sucrose present. This kit has a linear range of detection between 0.2–1.0 nmole of glucose for the fluorometric assay and 2–10 nmoles of glucose for the colorimetric assay.
This kit is suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.
Components
The kit is sufficient for 100 assays in 96 well plates.
- Glucose Assay Buffer 25 mL
- Glucose Probe, in DMSO 0.2 mL
- Invertase 1 vl
- Glucose Enzyme Mix 1 vl
- Sucrose Standard, 100 nmole/mL 0.1 mL
Reagents and Equipment Required but Not Provided.
- 96 well flat-bottom plate – It is recommended to use black plates with clear bottoms for fluorescence assays and clear plates for colorimetric assays.
- Fluorescence or spectrophotometric multiwell plate reader.
