ultrafleXtreme™
Experience Unmatched Sensitivity and Performance with Next-Generation MALDI-TOF and TOF/TOF Technology
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This is a wonderful piece of equipment. A great piece of equipment for analysis of proteins.
Protein and peptide analysis
Equipment has a user friendly interface. The software is easy to understand. Different experiments can be performed with this equipment. Very good results can be achieved.
Review Date: 24 Jan 2020 | Bruker Daltonics
Good results
biomarker discovery
Effective for application, easy to use, good customer support
Review Date: 13 Feb 2019 | Bruker Daltonics
High performance and flexibility for clinical and applied proteomics applications
Proteomics, MALDI-MSI, Mass Spectrometry Imaging
Bruker's Ultraflextreme MALDI-TOF is able to be used for conventional proteomic analyses as well as MALDI Mass Spectrometry Imaging. This versatile mass spectrometer is able to achieve 2 kHz speed in MS mode and 1 kHz in MS2 being able to analyse and process samples in a fast, reliable way. The UI of the software provided is user-friendly and, despite the parameter optimization being a complex procedure, the common user can achieve results after one month of training. I would definitely recommend it for laboratories interested in achieving consistent proteomics results: Bruker support is always available for troubleshooting and support. It's featured in countless publications, ranging from microbiology to plant proteomics, tumor heterogeneity and biomarker discovery studies.
Review Date: 31 Mar 2016 | Bruker Daltonics
Biotechnology, Proteomics
Two applications in particular, Imaging MS and Biotyper rank amongst top in comparison to all other models. You get the best quality results in the shortest time possible for those applications and it is pretty easy to program and to use in high-throughput applications. This comes highly recommended, especially since there has been an upgrade in the Biotyper identifications and the Smartbeam laser for higher resolution applications.
Review Date: 16 Oct 2015 | Bruker Daltonics
The instrument is easy to operate with detailed instructions in the manual, robust and stable. The software is user friendly. Service after sale is very good.
The product is best suited for imaging purpose in metabolomics and proteomics.
UltrafleXtreme is well suited for imaging and profiling of large biomolecules especially lipids, peptides and proteins. The instrument is easy to operate with detailed instructions in manual, robust and stable. The software is user friendly. Service after sale is very good. For European countries, service can be provided almost right away. Technicians from company can observe and check out the instrument via internet. Generally, this instrument is excellent for routine analyses in metabolomics and proteomics with spatial resolution of imaging not better than 20 micrometer.
Review Date: 28 Apr 2015 | Bruker Daltonics
Protemic & Glycomics reserarch
Very user friendly with a multipurpose interface. Can be used for peptides and proteins with extreme ease and reproducibility. Many user friendly options available for making it an easy to use machine. After sales care is ok and not up to the mark with a weak after sales service support. The machine is very robust and truly value for money. Overall the system is best suited for proteomics application.
Review Date: 31 Mar 2015 | Bruker Daltonics
Small Molecules
Bruker Utraflex MALDI-TOF/TOF-MS series are primarily designed for Proteomics application. It’s an outstanding easy to use instrument that works very stable for years long without significant problems and doesn't require many consumables. Flex analysis software generally required few minutes training to enable user to start acquiring spectra. Apart from Proteomics one by knowing the basics of LDI and TOF analyzers, can use instrument for the analysis of small molecules with extremely high throughput. Especially such instrument can be applied for species finger printing purposes with and without matrix approaches. It can deliver very sensitive and accurate measurements for small molecule if calibrated correctly. People working in areas like matrix development, matrix free LDI, solution free LDI can try many different options to efficiently ionize the molecules of interest but such application required advance knowledge of instrument design which is generally not given out by vendors!
Review Date: 22 Apr 2014 | Bruker Daltonics
Proteomics and Mass Spectrometry
Robust, easy to use, user friendly software. Compatible with diverse macro molecule applications.
Review Date: 5 Aug 2013 | Bruker Daltonics
Proteomics
The instrument is easy to use, has user friendly software and gives a good performance.
Review Date: 20 Jun 2012 | Bruker Daltonics
Easy to use, even for students.
Review Date: 18 Aug 2011 | Bruker Daltonics
Maximized Data Output: Cutting-edge technology enables the acquisition of complete MS/MS data sets at a rapid rate of up to 1000 Hz, even with low fmol levels, in a matter of seconds.
Precise Results: Advanced software analysis and visualization ensure accurate identification of high-content data, providing results with a high level of confidence.
Versatile Solution: The proprietary smartbeam-II laser provides unparalleled versatility, accommodating a range of analytical workflows and matrix options.
High-Speed MALDI-TOF/TOF Imaging of Mouse Brain Tissue Performed on Intact Proteins and After On-tissue Digestion
In this application note, the capability and flexibility of the MALDI-TOF/TOF system for imaging of intact proteins and tryptic peptides (after tissue digestion) is demonstrated. The on-tissue digestion approach benefits from significantly higher mass resolution and accuracy that can be achieved for tryptic peptides in reflector operation mode, as well as from aditional specificity provided by MS/MS analysis.
Full Validation of Therapeutic Antibody Sequences by Middle-Up Mass Measurements and Middle-Down Protein Sequencing
This application notes reports a novel middle-up LC-ESI-MS subunit analysis and middle-down LC-MALDI in-source decay (ISD) mass spectrometry method applied to cetuximab, panitumumab and natalizumab selected as representative FDA and EMA approved monoclonal antibodies (mAbs). The regulatory agencies require comprehensive primary sequence validation both for innovator and biosimilar monoclonal antibodies (mAbs). Full sequence coverage (SC) is typically obtained by the combination of multiple LC-MS/MS datasets from various protease digests.
High-Throughput Screening of Deubiquitylase Enzyme (DUB) Activity/Specificity and Inhibitor Screening by MALDI-TOF Mass Spectrometry
In biopharmaceutical laboratories, there is a growing need for high-throughput, yet sensitive methodologies to characterize deubiquitylase enzymes (DUBs) and DUB inhibitors. This application note demonstrates a sensitive and fast assay to quantify in vitro DUB enzyme activity using matrix-assisted laser desorption/ionization time-of-flight (MALDITOF) mass spectrometry, called MALDI-TOF DUB assay.
High Resolution Lipid Profiling and Identification by Hyphenated HPTLC-MALDI-TOF/TOF
This application note demonstrates HPTLC-MALDI-MS/MS analysis of lipid mixtures, a technique that provides additional structural information on headgroup and acyl chain composition and enables detailed assignment of lipid structures, even if mixtures are only partially resolved by the chromatographic separation.
An Immuno-Mass Spectrometric Assay for Quantification of Soluble Transferrin Receptor in Human Serum
This poster describes an automated approach for quantification of endogenous soluble transferring receptor in human serum as a model system for the new generation of immune-mass spectrometric assays.
Comprehensive Study of O-linked Glycopeptides of Erythropoeitin
Protein glycosylation can effectively be analyzed on the glycopeptide level where site-specific modifications remain preserved. This application note introduces a new method allowing for automatic identification of unknown glycopeptides in one step without prior release of the glycans. MALDI-TOF/TOF technology combined with an integrated software solution is used to analyze and compare O-linked glycosylation of two erythropoietin samples of different origin. MALDI-specific fragmentation patterns are used to successfully find the glycopeptide spectra in a complete LC-MALDI dataset and identify glycan and peptide moieties.
Automated Acquisition of MALDI-ISD Spectra for the N- and C-terminal Sequence Determination of Intact Proteins
For over 10 years, MALDI-Top-Down Sequencing (MALDI-TDS) of intact proteins has proved particularly useful for the determination of N- and C-terminal protein sequences. This technology note describes a procedure using the recently introduced 1,5-diaminonaphthalene (DAN) matrix and the new ultrafleXtreme™ MALDI-TOF/ TOF that automates the entire analysis - from sample preparation and data acquisition to N- and C-terminal sequence assignments.
Precise MW Determination of Intact Proteins by Multiple Charge State Analysis of MALDI Generated Ions
This application note describes a new approach for the precise MW determination of intact protein proteins using multiple charge state analysis of MALDI generated ions. The combination of the new ultrafleXtreme with Bruker’s DHAP matrix provides very high accuracies for the MW determination of proteins in the 8-70 kDa range in a unique new concept of tracking advantage of highly resolved and multiple charged ions in a precisely calibrated m/z range.
Characterization of the N-glycosylation Pattern of Antibodies by ESI - and MALDI Mass Spectrometry
This application note describes the in-depth characterization of N-glycosylation pattern in various antibody samples using two complementary mass spectrometric strategies based on both, top-down ESI-UHR-TOF and bottom-up LC-MALDI-TOF/TOF performed on the Bruker maXis™ and ultrafleXtreme™ platform, respectively.
BioPharma Accurate Intact Protein Mass in 5 Seconds with ultrafleXtreme™
This application brief describes the analysis of protein mass using the Bruker ultrafleXtreme™. Results show that the ultrafleXtreme™ obtains ppm mass accuracy for proteins up to 70 kDa in 5 seconds, with proteins up to 30 kDa being isotopically resolved.
Introducing the New ultrafleXtreme MALDI-TOF Mass Spectrometer from Bruker
Watch this video to learn about the new ultrafleXtreme from Bruker. Discover how the features of the ultrafleXtreme allow you to attain the highest level of mass spectrometry performance and enable extreme flexibility for a broad variety of complementary research, clinical and applied proteomics applications.
Webinar Highlights - Label-Free High-Throughput MALDI TOF Mass Spectrometry in Drug Discovery
Learn more about the high-throughput screening of biochemical assays using MALDI TOF and its use as a tool for drug discovery
Analysis of Biosimilars and Biobetters using the maXis UHR-qTOF: A Complementary Approach
Written by Jason S. Wood, Ph.D. Bruker Daltonics
