ResourceSpectroscopy
Automated Acquisition of MALDI-ISD Spectra for the N- and C-terminal Sequence Determination of Intact Proteins
24 Apr 2013For over 10 years, MALDI-Top-Down Sequencing (MALDI-TDS) of intact proteins has proved particularly useful for the determination of N- and C-terminal protein sequences. This technology note describes a procedure using the recently introduced 1,5-diaminonaphthalene (DAN) matrix and the new ultrafleXtreme™ MALDI-TOF/ TOF that automates the entire analysis - from sample preparation and data acquisition to N- and C-terminal sequence assignments.
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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
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