Optima MAX-XP

Single walled carbon nanotubes experienced increasing interest in the past 15 years for semiconductors, fuel cells and biomedical applications. This application note focuses on two challenging areas for nanoparticles: reliable, rapid scale-up of nanoparticle purification; and quantitative analysis of the concentration of species present. The Biomek 4000 Laboratory Automation Workstation helps overcome the human variable and gives a consistent, reproducible, high-throughput method for gradient setup, which provides a breakthrough for scale-up problems.

This application note provides insight into the inherent shortcomings that are present in traditional cellular toxicity assays, and how these shortcomings are magnified when the toxicity assay involves nanomaterial. Nanomedicine is a field common to materials scientists, chemists, biomedical engineers, biologists and medical scientists. Beckman Coulter tools enable quantification of cellular toxicity caused by nanoparticles; reducing time to remove aggregated particles and quantifying cell toxicity in the presence of single-walled carbon nanotubes.

This application note describes the characterization of a heteroassociating system. The thermodissociation is studied using band sedimentation analysis, whilst the electrostatic dissociation is studied using boundary sedimentation analysis. Analytical ultracentrifugation represents a powerful methodology for expressing HIV-1 viral components. In conjunction with appropriate data analysis methodologies, analytical ultracentrifugation can be used to examine sample purity, detect and characterize conformational changes, determine subunit stoichiometries, characterize assembly and disassembly mechanisms of macromolecular complexes, and measure equilibrium constants and thermodynamic parameters of associating systems.

Optima AUC delivers enhanced optics and improved software to open new applications