compact QToF

Persistent Organic Pollutants (POPs) are major concern for the environment, quantifying them with sensitivity and confidence increasingly important. The study in this application note uses an atmospheric pressure chemical ionization GC coupled to a high-resolution QTOF – MS to analyze the levels of key substances in POPs, including decaBDE which is the most difficult PBDE to analyze.

Protein glycosylation is a dynamically regulated, non-template dependent process influenced by various factors such as age, sex, cell and tissue type as well as health status. This makes glycoproteins prime biomarker targets. In order to investigate the role individual glycoproteins play in the onset and progression of numerous diseases reliable and robust analytical approaches are crucial for determination of glycan composition in a protein/peptide specific manner. Mass spectrometry has developed as one of the most powerful tools for the sensitive identification and structure elucidation of glycopeptides. However, the optimal collision energy conditions required to obtain a maximum of information on both, the glycan as well as the peptide moiety have to date rarely been systematically investigated.

Soft ionization modes in GC-hyphenation studies such as chemical ionization (CI), field ionization (FD), supersonic molecular beam (SMB), or atmospheric-pressure chemical ionization (APCI) allows the effective formation of pseudo-molecular ions from biomolecules. GC-APCI generic source coupling is one of the more promising method as it is dually compatible with both GC and LC-hyphenated mass spectrometers. In this study, the optimization and development of a dopant-based APCI screening workflow using next-generation GC-APCI ion source on a QTOF platform for multi-target pesticide screening is presented using the Bruker Compact™ QTOF system.

This poster demonstrates a method to extend the specificity of intact mass analysis and Middle-Down sequencing by deploying MD MALDI-ISD for high quality identification of sequence variations in combination with a new ultrahigh resolution (UHR)-ESI QTOF providing monoisotopic mass determinations of HCs with no speed or dynamic range compromise.

A previous study demonstrated that non-targeted metabolomics enabled differentiation of coffee types based on their assigned flavor intensity and identified trigonelline (N-methyl-nicotinic acid) as one characteristic compound for weak coffee. This poster demonstrates a novel workflow for a pathway driven targeted metabolomics approach based on the same high resolution LC-QTOF data files used for the initial non-targeted profiling.

The application note uses the Compass PathwayScreener™ to enable mining the same full-scan high resolution LC-MS data set used for an initial non-targeted metabolomics workflow in a pathway-driven targeted fashion.

Learn how to improve your data acquisition in high-resolution mass spectroscopy

Unique trapped ion mobility spectrometry (TIMS) coupled to Bruker’s high-performance QTOF mass spectrometry enables high-resolution compound separation, significant duty cycle enhancements and studies of protein conformations, aggregation and structural isomers

Novel MetaboScape software links experimental data to biology by pathway mapping