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Methods for improved cell counting accuracy

DeNovix
24 Feb 2025

Trypan Blue is widely used in cell culture labs for selectively staining dead cells and tissues. However, some sample types can present challenges for accurately assessing cell counts and viability with Trypan Blue. For these sample types, a fluorescence assay such as Acridine Orange / Propidium Iodide (AO/PI) is a simple and highly accurate alternative. Learn about each viability method and the situations where fluorescence is advantageous in this free eBook.

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CellDrop™ Automated Cell Counters

DeNovix

Reduce consumable costs and improve lab sustainability with patented DirectPipette™ technology, or use slide mode for ultimate measurement flexibility. The CellDrop features dual fluorescence and brightfield optics, variable height sample chamber to make cell counting fast, simple, and reproducible.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.Fluorescent Dyes

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Methods for improved cell counting accuracy