Whole Genome Amplification Service from LGC

24 Dec 2013
Sarah Thomas
Associate Editor

Product news

Whole Genome Amplification (WGA) is a PCR technique that is used to produce large quantities of DNA from a small amount of starting material. Unlike conventional PCR, WGA is aimed at amplifying the entire genome of an organism rather than a specific region. LGC is offering their Whole Genome Amplification Service from £4.68 / €5.61 / $8.98 per sample, until the end of January. The total price is dependent on final number of samples. Please request a quote for your project specific price.

How much input DNA do I need to provide for WGA?

A minimum mass of 50ng DNA is required per reaction. Note: if the genomic DNA is from a species with a genome size considerably larger than that of human, the required starting mass of DNA will be greater than 50ng.

What yield can be expected?

The product is usually amplified to a concentration of 500-1000 times that of the starting material. Actual yield is dependent on quality of input DNA. The DNA is suitable for many applications including KASP genotyping on more than 1000 SNPs.


Why choose LGC?

• Convenient and fast DNA amplification.
• DNA ready for many applications, including KASP™ Genotyping on thousands of SNPs.
• Our experienced scientists work with our customers in AgBio, academics, government research and healthcare industries every day to solve difficult challenges.

More information is available at http://www.lgcgenomics.com/genotyping/whole-genome-amplification
Request your project specific quote here.

Links

Tags

Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.Whole Genome Amplification