KASP™ Genotyping Reagents
Unmatched quality, reliability and credibility have established KASP as the foundational SNP genotyping chemistry for the Agrigenomics industry.
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The patented KASP™ genotyping method is a homogeneous, FRET-based end-point PCR assay that enables accurate bi-allelic discrimination of known SNPs and InDels.
Unlike other PCR-based genotyping assays, KASP requires no labelling of the target-specific primers or probes, giving it a clear cost advantage. It also allows greater flexibility for assay design, which translates into a higher overall success rate and the unique ability to genotype large/mega InDels. For over 20 years, the KASP assay has been cited in over 2,500 peer-reviewed publications, developed into tens of thousands of pre-validated assays across many commercially important crops, and used to develop over 200,000 SNP genotyping assays in more than 75 organisms, including higher ploidy plant species.
Whether purchased as a product for use in your laboratory, or as a genotyping service at our state of the art genotyping laboratories in Europe and North America, KASP genotyping delivers accurate results and significant cost savings so customers can get the most out of their research budget. KASP genotyping is based on an open format PCR workflow. There is no need to buy new expensive equipment to be able to run KASP in your own laboratory; completed KASP reactions can be read on most FRET-capable plate readers and qPCR instruments.
Features:
- Accuracy: >99.8% based on independent assessment
- Industry leading SNP & InDel assay conversion rate (>90%)
- Flexible primer design which increases the rate of successful assay development
- Supports low, medium and high-throughput studies and individual repeat assays
- Compatibility with a wide range of liquid handling systems and thermal cyclers, signal can be read on most FRET-capable plate readers and qPCR instruments
- Breakthrough cost savings
Applications:
- Many applications in plant, animal, human and microbial research
- Plant Marker-assisted selection for crop improvement (including MARS and MABC)
- Fingerprinting / parental screening
- SNP validation
- Field trial genotyping
- Animal Marker-assisted selection for livestock breeding
- Speciation
- Sample provenance and quality control
- Mapping traits within disease models and breeding populations (e.g. zebrafish, mouse, rat)
- Speed congenics
- Human Genetic variant analysis for human disease research
- Cohort studies
- Disease SNP replication/validation studies
