NewsSeparations

The New SelectScience HPLC Columns Buying Guide is Out Now!

SelectScience

Save valuable time with this exclusive guide to HPLC column technology

6 Aug 2015

Lois Manton-O'Byrne, PhD

Executive Editor

Editorial article

Save valuable time with this exclusive guide to HPLC column technology

Download this guide for instant access to all the information you need to make the right purchasing decisions for your laboratory. Learn about application considerations, column properties, performance criteria and the future for HPLC columns.

Buying Guide Contents

Basic Overview

Application Considerations

Stationary Phase Considerations

Column Chemistry Considerations

Separation Mode Considerations

Particle Property Considerations

Retention Capacity Considerations

Column Dimension Considerations

Future Trends

Summary

You will also find independent, impartial reviews of laboratory equipment to help you buy with confidence:

"This column does a better job of resolving our analytes from matrix constituents than the traditional C18..."

"Good separation and resolution; fast, reliable and accurate..."

Download the Buying Guide now >>

Related products

Request Quote for All Products

Chromolith® High Resolution HPLC Columns
Merck KGaA, Darmstadt, Germany
Chromolith® HighResolution: High resolution separations without ultra high pressure. The revolutionary monolithic Chromolith® HighResolution columns are made from a single rod of high-purity monolithic silica, rather than from particles. Their unique bimodal pore structure allows the chromatographic performance to be improved significantly. Speed of analysis (separations up to 9 times faster if required) Separations two times faster at half the column back-pressure compared to μm columns Higher sample throughput Improved HPLC system security Significantly increased column lifetime Reduced maintenance on HPLC pump and injector seals Reduced need for sample preparation as columns are very resistant to blocking (even with biological samples) Column length no longer pressure limited Very high separation efficiency by column coupling
(14)
Raptor™ C18 LC Columns (USP L1)
Restek Corp.
Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or “core-shell” particles) with the resolution of highly selective USLC® technology. This new breed of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation.
(2)
ACQUITY UPLC® BEH C18 and C8 Columns
Waters
ACQUITY UPLC® BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC® separations by providing the widest pH range.They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. This low pH stability is combined with the high pH stability of the 1.7 µm BEH particle to deliver the widest usable pH operating range. In addition, these new chemistries also utilize new, proprietary endcapping processes which produce outstanding peak shape for bases. These bonding chemistries and particle synthesis innovations produce the sharpest peaks, highest efficiencies and maximum MS sensitivities.
(15)
Poroshell 120
Agilent Technologies
Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC analyses, and can accelerate your older, traditional 5 µm method for 4-5x reduction in analysis time. With a family of ten chemistries, including the recently introduced PFP, and alignment with the ZORBAX family chemistries, method development and transfer is flexible and easy.Features: High efficiency and high resolution, with up to 50% less backpressure than sub-2 μm columns 2 μm frit, for rugged performance with dirty samples Compatible with 400 bar and 600 bar LCs, as well as UHPLC instruments An expanding family of bonded phases to align with the ZORBAX Family, for reliable scalability Excellent selectivity and peak shapes Designed for exceptional reproducibility Explore Poroshell 120 solutions focused applications, from food to pharma
(18)
Raptor™ ARC-18 LC Columns
Restek Corp.
The Raptor™ ARC-18 column is made specifically for high-throughput, low–sample prep LC-MS/MS applications. A well-balanced retention profile offers better detection and integration of large, multiclass analyte lists, and steric protection helps this SPP column endure the harsh, low-pH mobile phases needed for mass spec without sacrificing retention or peak quality.
(10)