Poroshell 120
Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC an…
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Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.
Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC analyses, and can accelerate your older, traditional 5 µm method for 4-5x reduction in analysis time. With a family of ten chemistries, including the recently introduced PFP, and alignment with the ZORBAX family chemistries, method development and transfer is flexible and easy.
Features:
- High efficiency and high resolution, with up to 50% less backpressure than sub-2 μm columns
- 2 μm frit, for rugged performance with dirty samples
- Compatible with 400 bar and 600 bar LCs, as well as UHPLC instruments
- An expanding family of bonded phases to align with the ZORBAX Family, for reliable scalability
- Excellent selectivity and peak shapes
- Designed for exceptional reproducibility
- Explore Poroshell 120 solutions focused applications, from food to pharma
Analysis of oligonucleotides using an ion-pairing-free reversed-phase method with TOF LC/MS
The most common method for the analysis of synthetic oligonucleotides is ion-pairing reversed-phase (IPRP) however, it is costly, environmentally harmful, and requires dedicated instrumentation. Explore an alternative method that uses time-of-flight liquid chromatography/mass spectrometry (TOF LC/MS) and a reversed-phase (RP) chromatographic method which enables accurate and repeatable analysis of diverse oligonucleotides without the use of ion-pairing reagents.
Emerging alternative analytical techniques for oligonucleotide separation and purification
As the therapeutic oligonucleotide market continues to mature, the need for alternative analytical approaches emerge to meet those demands. The current standard widely used for oligonucleotide analysis is ion-pairing reversed-phase chromatography (IP-RP) and ion-exchange chromatography (IEX). However, these instruments come with limitations such as, incompatibility and flexibility.
Explore an effective and emerging technique for oligonucleotide workflows – hydrophilic interaction chromatography (HILIC) and size exclusion chromatography (SEC) – and uncover the benefits it can provide for your separation and purification of oligonucleotides.
