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Discover the Latest Technologies and Methods for Cell-Based Assays and In Vitro Analysis

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New application notes, videos and product

10 Aug 2015
Lois Manton-O'Byrne, PhD
Executive Editor

Editorial article

New application notes, videos and product

Learn about innovative methods for analyzing 3D tumor invasion and cytotoxicity, and discover how atmospheric control can benefit your long-term assays.

1. APPLICATION NOTE: Assessing Drug Residence Time and Anti-Metastatic Effects of CXCR4 Inhibitors Through Kinetic Ligand Binding and 3D Tumor Invasion

The expression of chemokine receptors, such as CXCR4 and CCR7, is tightly correlated with the metastatic properties of breast cancer cells. This study demonstrates the use of the Synergy™ Neo and Cytation™ 5 Cell Imaging Multi-Mode Readers to provide PMT-based assessment of drug-target residence time of various CXCR4 inhibitors, as well as automated image-based monitoring of tumor invasion through a basement membrane matrix. Download method.

2. PRODUCT: Pall ForteBio Launches the Octet® K2 System, Increasing Access to Sensitive Binding Interactions Analysis

Pall ForteBio LLC announced the launch of the Octet K2® system, a new entry point into the Octet line of label-free interaction analysis systems. Now available globally, the Octet K2 system offers high-performance, budget-friendly kinetic characterization of antibody, protein and small-molecule binding interactions. Read more.

3. APPLICATION NOTE: Multiparametric Analysis of Cytotoxicity using Cell Imaging in Combination with Luminescence

During the drug development process, where cytotoxicity remains one of the major causes of drug withdrawal, there is an urgent need for reliable and time-saving assay workflows that gain a more complete picture of the effects of cytotoxicity. This application demonstrates how multiparametric analysis of cytotoxicity can be easily performed on the EnSight™ Multimode Plate Reader. The study shows brightfield confluency analysis to serve as a robust indicator for toxicity in adherent cells in labeled or label-free cell-based assay. Download method.

4. APPLICATION NOTE: New CLARIOstar® Atmospheric Control Unit for Versatility in Long Term Cell-Based Assays

This application note demonstrates how CLARIOstar®'s new Atmospheric Control Unit (ACU) allows time- and dose-dependent compound effects on cell proliferation and cytotoxicity to be measured directly in the cell culture. Download method.

5. PRODUCTS: Top Microplate Readers as Reviewed by You

Discover some of the top rated microplate readers as reviewed by SelectScience members. Read what your scientific peers think about the equipment they are using and which products they recommend. Read more.

6. VIDEO: Standardizing Secondary Phenotype Image Analysis with PerkinElmer’s High Content Profiler

Dr. Christopher Hale from Amgen is committed to unlocking the potential of biology for patients suffering from serious illnesses by discovering, developing, manufacturing and delivering innovative human therapeutics. In this video, Dr. Christopher Hale advocates the use of PerkinElmer’s High Content Profiler which enables him to standardize his workflow, run quality control on data sets, run principal component analysis and much more. He also discusses how his work is made possible thanks to PerkinElmer’s Operetta and Opera Phenix high content screening systems, which are both easy-to-use and suited to long term assays and confocal imaging. Watch video.

7. APPLICATION NOTE: Phenotypic Screening and In Vitro Toxicology: Applications of Far‐Red Fluorescing DNA-Specific Anthraquinones in Cell‐Based Assays

This application note demonstrates a number of applications of Fluorescein-/GFP-compatible dyes, in particular DRAQ5 and CyTRAK Orange. The particular spectral and chemical features of DRAQ5 and CyTRAK Orange mean they are compatible with aqueous media and fixatives. Conveniently, they are supplied in aqueous solution and can be applied as the final step in a sample preparation. They have become standard reagents for immunofluorescence microscopy, flow cytometry and high content imaging. Download method.

8. PRODUCT: Highly Efficient ChIP Assay Kit for FFPE Samples

Chromatrap®, a business unit of Porvair Sciences, has introduced a new Formaldehyde Fixed Paraffin-Embedded (FFPE) ChIP Kit which overcomes the widely acknowledged shortfalls of the technique. The new Chromatrap® FFPE ChIP kit works equally well with high or low abundant marks and provides sufficient DNA to perform library preps for ChIP-seq assays. Read more.

Have you used any of these products? Share your experiences and write a review today!

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Agilent BioTek Synergy Neo2 Hybrid Multimode Reader

Agilent Technologies

Agilent BioTek Synergy Neo2 multimode microplate reader features proprietary Hybrid Technology, with independent optical paths that ensure excellent performance in all detection modes. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF, and up to 4 PMTs provide ultrafast measurements.

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Chromatrap® FFPE ChIP Kit

Chromatrap

Epigenetic identification and targeting in clinical FFPE tissues is a crucial step for potential epigenetic therapeutics and personalized medicine therapies. FFPE is a standard method to archive and preserve medical tissue biopsy samples. These archives, kept in many hospitals world-wide, are a huge, rich and untapped sample resource for genetic research. Due to the cross-linking of proteins by formaldehyde, FFPE tissues present a particular challenge for ChIP analysis. In addition, the small size and delicate nature of the tissue, and the difficulty in extracting samples from paraffin, used to provide tissue support after preservation, can damage complex cellular complexes such as chromatin. ChIP assays from these samples have therefore proved time consuming and difficult.  Chromatrap® FFPE ChIP kit overcomes these issues and provides the following benefits: Provides greater flexibility and more IPs per sample Works across a range of FFPE samples, human and animal Available in 96-well plate or spin column format Works equally well with high and low abundant marks Provides sufficient DNA to perform library preps for ChIP-seq assays

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MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.CytotoxicityCytotoxicity assays measure the toxic effects of substances on cells, often used in drug testing and environmental studies. These tests are crucial in determining the safety of chemicals and pharmaceutical compounds. Explore cytotoxicity testing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.TumorsTumor research focuses on understanding abnormal cell growth that leads to cancer. Identifying biomarkers, studying tumor microenvironments, and developing targeted therapies are critical for advancing cancer treatment. Early detection and personalized treatment options are key to improving outcomes for patients. Browse our peer-reviewed product directory to explore tools for tumor research, diagnostics, and cancer therapies; compare products, read customer reviews, and get pricing directly from manufacturers.LuminescenceLuminescence techniques detect and measure light emitted by substances during chemical reactions or biological processes. Widely used in assays for diagnostics, environmental monitoring, and drug screening, luminescence-based methods offer high sensitivity. Explore leading systems and products in our directory.Phenotypic ScreeningPhenotypic screening assesses cellular responses to compounds, enabling drug discovery and target identification. This technique is pivotal in finding effective treatments for complex diseases. Discover phenotypic screening platforms and tools with peer-reviewed comparisons and pricing in our directory.Cell BiologyCell biology studies the structure, function, and behavior of cells. Understanding cellular processes is essential for research in areas such as cancer biology, stem cell research, and neurobiology. Techniques like flow cytometry, microscopy, and cell culture enable researchers to explore cellular mechanisms in detail. Browse our peer-reviewed product directory to find the best cell biology tools and equipment, compare products, check customer reviews, and get pricing directly from manufacturers.

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