Transcreener CD73 assay: Leveraging nucleotide detection to measure adenosine
7 Jun 2020
Watch this webinar from Meera Kumar, Senior Scientist at BellBrook Labs, to learn more about CD73 as a target for immunotherapy, including information on how the transcreener CD73 assay was developed and how this assay can be used to help measure adenosine.
About the company
BellBrook Labs
BellBrook Labs- Screening Tools for the Pharmaceutical Industry
BellBrook Labs is dedicated to providing the pharmaceutical industry with enabling screening tools to accelerate the discovery of more effective therapies.
As a small, R&D-driven company, Bell Brook Labs identify areas where there is a strong need but a significant technical hurdle, and then we develop a solution. BellBrook is focused on taking creative approaches to new product development and to continually improving the level of service provided to our customers. When you communicate with someone from BellBrook it will be immediately apparent that they share your passion for discovering more effective disease therapies and your respect for the complexity of biology.
BellBrook Labs specializes in two major technology platforms-
Transcreener® Biochemical Assay Technology:
Universal, high throughput screening platform for enzymes based on direct detection of nucleotide enzyme products with far red FI, FP and TR-FRET readouts. Transcreener assays enable you to screen more targets, both well characterized and emerging, faster and more efficiently.
• Four assays cover thousands of target enzymes, including any kinase, ATPase, or GTPase.
• Direct detection means less chance for compound interference.
• Far Red FP, FI and TR-FRET readouts validated on major multimode readers.
• Single step, mix and read format with 8 hour deck and signal stability for easy automation.
Related products
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Assay Development Services
BellBrook LabsBellBrook Labs delivers bespoke assay development and lead discovery solutions using its Transcreener® HTS platforms. Their expert team optimizes detection of small-molecule modulators for enzyme targets—tailoring buffer, enzyme, and detection conditions to yield strong initial-velocity signals (Z’ 0.7–0.9) in just 2–4 weeks.
Transcreener® ADP² Kinase Assays
BellBrook LabsThe Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.
Transcreener® AMP²/GMP² Phosphodiesterase Assays
BellBrook LabsThe Transcreener® AMP²/GMP² Phosphodiesterase Assay Kit directly detects AMP or GMP produced by PDEs, ligases, synthetases, ENPP1, and related enzymes using a mix‑and‑read FP or TR‑FRET format—no coupling steps needed. Compatible with 1–1000 µM native substrates, it delivers excellent HTS performance (Z’ ≥ 0.7, ≥100 mP shift) in a one-step, far‑red fluorescence assay.
