Development of a High Throughput Method for the Screening of a Maize Mutant Collection HD

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis.  KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.   Quantitate changes in gene expression more accurately High reaction efficiency between 95–105% improves accuracy and reproducibility Unbiased efficiency across a wide range of GC contents and amplicon lengths Detect low copy and difficult targets consistently Improved processivity results in earlier Cq scores Higher fluorescence detection across varying AT- and GC-rich targets Novel enzyme is resistant to SYBR inhibition Complete real-time PCR runs in just 40 minutes 55% shorter run times with fast cycling protocol Maintain high performance when switching from standard to fast protocols   Applications: Gene expression Gene knockdown validation Microarray validation ChIP analysis Low copy detection Absolute quantification of NGS libraries

KAPA3G Plant PCR Kits are designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR). In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR). The KAPA3G Plant PCR Kit contains KAPA3G DNA Polymerase, a novel, third-generation (3G) enzyme which was engineered via a process of directed evolution for improved tolerance to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides. The KAPA3G Plant PCR Kits are optimized for fast and efficient amplification of plant DNA from crude samples, DNA-containing carry-over inhibitors from crude extraction methods, and purified DNA.   Features: Amplify long targets from crude samples and purified DNA Amplify fragments up to 5 kb from purified DNA and crude samples High yield and specificity with purified DNA and crude samples Perform direct PCR from a variety of plant species and tissue types Direct PCR with leaf disc or seed as template No need for time-consuming DNA extractions Streamline workflows and improve turnaround times Perform PCR in half the time compared with wild-type enzymes Eliminate the need for time-consuming DNA extractions Improve success rates with novel crude sample plant PCR workflow Use extraction buffer to prepare crude extracts for plant PCR in just 5 minutes High success rates with even the most challenging sample types   Applications: Amplification of fragments up to 5 kb in size from purified plant DNA, extracted with commercial kits or CTAB-based methods Direct PCR from leaf discs, seed samples, and other plant tissue types PCR from crude plant DNA extracts, prepared from leaf and/or seed material