Automated Two-Step Purification of Mouse Antibody IgG1
11 Jan 2018
Dr. Yannick Krauke from the KNAUER lab describes an automated purification of mouse immunoglobulin antibodies (IgG) from cell culture. The cell culture was applied with a feeding pump on a proteinA affinity column to capture and purify the antibodies. These were kept in the system and in a second step applied on a gel filtration column for buffer exchange. The obtained antibody was now dissolved in the desired storage and/or working buffer. This application note highlights the possibility of automated purification with the AZURA BIO LC system without manual interaction during purification process.
About the company
KNAUER Wissenschaftliche Geräte GmbH
We separate molecules and unite people.
Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:
- Analytical HPLC / UHPLC
- Preparative HPLC
- Fast protein liquid chromatography (FPLC)
- Multi-Column Chromatography / Simulated moving bed (SMB)
- Osmometry
With 135 employees, we are an established manufacturer of HPLC systems and components. Our valves, pumps, detectors, and other components are used for many separation processes outside of HPLC and are popular with customers who want custom solutions (OEM). KNAUER is an ideal partner for those who want more than standard solutions can provide. Osmometers for the determination of molality were the first products of the family business and the cornerstone of its success. KNAUER continues to develop and manufacture industry-leading osmometers.
Furthermore, the KNAUER Academy offers courses on HPLC and application development.
We support technological advancement today and in the future.
