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Automated Purification of DNA, RNA and miRNA from FFPE Tissue

29 Apr 2021
Automated Purification of DNA, RNA and miRNA from FFPE Tissue

Researchers and clinicians continue to be challenged by FFPE tissue samples with a low abundance of high-quality nucleic acid. FFPE samples frequently fail to produce DNA or RNA with sufficient yield or quality for downstream analyses when conventional extraction methods are used. Despite efforts to optimize these labor-intensive extraction methods, extracts from FFPE samples frequently fail to qualify for next-generation sequencing or fail to yield actionable results when sequenced. In this webinar, we will review data from samples purified on the Ionic® Purification System that demonstrates the ability of the system to recover DNA, RNA, or miRNA from challenging FFPE tissue samples with sufficient yield and quality for downstream molecular analyses.

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Purigen Biosystems

Purigen Biosystems' transformative Ionic Purification System provides a hands-free solution for extracting, enriching and quantifying DNA and RNA from biological samples. Our proprietary approach uses isotachophoresis (ITP), an electric-field-driven technique for purifying, focusing, and/or separating species. The application of ITP to purify and quantify nucleic acids from complex biological samples was pioneered by Purigen co-founder, Stanford Professor Juan G. Santiago. The Ionic® Purification System is compatible with a range of samples including cells and FFPE. Purigen is proudly affliated with StartX Med, a Stanford-University-affliated nonprofit technology accelerator, and the California Life Sciences Institute’s (CLSI) FAST Advisory Program.

Ionic® Purification System

Purigen Biosystems

The Ionic® Purification System is a benchtop platform that provides automated extraction of DNA and RNA from biological samples. Using cutting-edge technology based on isotachophoresis, the Ionic system separates DNA and RNA freely in solution, without binding to or stripping nucleic acids from physical surfaces. The workflow is simple and efficient, while producing higher yields with higher purity, consistently and reliably.

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Automated Purification of DNA, RNA and miRNA from FFPE Tissue