Amplification and Detection of Foreign DNA
1 Sept 2016
Food & beverage labs often need to verify the absence of foreign DNA (bacteria, GMO, etc.) in food samples. In this workflow, the step of amplification and detection of the DNA is crucial. Watch this video to discover how to get correct results quickly to detect foreign DNA.
About the company
Eppendorf
Eppendorf is a leading life science company that develops and sells instruments, consumables, and services for liquid handling, sample handling, and cell handling in laboratories worldwide. Its product range includes pipettes and automated pipetting systems, dispensers, centrifuges, mixers, spectrometers, and DNA amplification equipment as well as ultra-low temperature freezers, fermentors, bioreactors, CO2 incubators, shakers, and cell manipulation systems. Consumables such as pipette tips, test tubes, microliter plates, and single-use bioreactor vessels complement the range of highest-quality premium products.
Eppendorf products are most broadly used in academic and commercial research laboratories, e.g., in companies from the pharmaceutical and biotechnological as well as the chemical and food industries. They are also aimed at clinical and environmental analysis laboratories, forensics, and at industrial laboratories performing process analysis, production, and quality assurance.
Eppendorf was founded in Hamburg, Germany in 1945 and has about 4,500 employees worldwide. The company has subsidiaries in 26 countries and is represented in all other markets by distributors.
More information about Eppendorf can be found at http://corporate.eppendorf.com/en
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Pipette tips - ep Dualfilter T.I.P.S.
EppendorfThere are certain situations in the lab in which suitable safety precautions must be taken in counteract any potential risks and to prevent any unpleasant surprises. It must be possible to anticipate in advance the dangers presented by carryover effects from radioactive, highly toxic or infectious sample material as well as the contamination risk caused by foreign DNA (e.g. as a result of aerosol formation).Our ep Dualfilter T.I.P.S. have an integrated dual filter made of pure hydrophobic polyethylene without "self-sealing" additives. This filter provides protection against carryover and contamination caused by aerosols and does not swell up as a result of incorrect pipetting. Samples can then be easily recovered. In line with the special biological purity requirements of a PCR application, ep Dualfilter T.I.P.S. are "PCR clean", sterile (in accordance with USP, DAB and Ph.Eur.) and thus free of human DNA, DNase, RNase and PCR inhibitors. The new Eppendorf ep Dualfilter T.I.P.S. are the first filtertips with a two phase filter for contamination protection. The tips are manufactured to the highest possible quality from pure, non recycled materials under cleanroom conditions. The are characterized by defined flow dynamics, low wettability and high thermal stability. They are certified pyrogen free and PCR clean (free from human DNA, DNase, RNase and PCR-inhibiting substances). The two-phases effect! Two filter layers provide dual protection for pipette and sample Two filter layers provide dual protection from aerosols and biomolocules Free from PCR inhibitor additives Patent pending technology All tips are provided sterile, Eppendorf PCR clean and pyrogen-free Batch certified ep Dualfilter T.I.P.S. contains well-defined different pore sizes within the two filter layers. The white layer which faces the sample retains drops, splashes and aerosols. the blue layer above it forms a highly-effective double barrier, that reliably binds even biomolecules. Even more, ep Dualfilter T.I.P.S. are characterized by: Defined flow dynamics Low wettability High thermal stability Leak proof seal* Minimum attachment and ejection forces* *when used in combination with Eppendorf pipettes
