ResourceDrug Discovery & Development
USP-compliant analysis of antibody drugs using size-exclusion chromatography
6 Feb 2025Proteins aggregate due to variances in temperature, pH, and concentration during manufacturing process and storage. Since aggregation of protein products such as monoclonal antibodies (mAbs) may affect safety and efficacy, the degree of aggregation of the products is confirmed by dedicated analytical procedures. Explore how Nexera™ lite inert, high-performance liquid chromatograph (HPLC) was used to perform USP-complied size exclusion chromatography (SEC) analysis of monoclonal IgG antibodies. It was able to provide good repeatability of analyses even employing mobile phase containing high concentrations of salt.
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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Antibody Drug Conjugates