Using the Waters Forensic Toxicology Screening Application Solution with UNIFI to Determine Diuretics in Urine

20 Jul 2015

Diuretics are a class of pharmaceutical compounds whose primary aim is to promote urine production. They can also be used to mask the presence of banned or illegal substances by facilitating dilution of the urine and aiding excretion. Consequently the use of diuretics is prohibited for athletes by the World Anti-Doping Agency (WADA). However, a number of diuretics only ionise in negative electrospray mode (ESI-). This application note investigates methods to further extend the Forensic Toxicology Screening Application Solution with UNIFI to include compounds that ionise in negative mode and to use the method to determine the presence of diuretics in urine, particularly at concentrations below the WADA MRPL.

Related products

Request Quote for All Products

ACQUITY UPLC® I-Class PLUS System
Waters
The most powerful solution to the most critical need in separation science today.
(2)
Xevo G2-XS ToF
Waters
Xevo G2-XS Tof - Performance, robustness, and flexibility made accessible to everyone. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the Xevo G2-XS Tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information, flexibility to adapt to changing needs, and is accessible to experts and non-experts alike.
(1)
Forensic Toxicology Screening Solution with UNIFI
Waters
Purpose-built Solution Achieves Reliable Results with Unparalleled Efficiency and Accuracy The Forensic Toxicology Screening Application Solution with UNIFI delivers the sensitivity, reproducibility, and ease-of-use necessary to perform broad screening techniques on complex biological samples to identify drugs of abuse and other toxicants of interest. Now, for the first time, you will be able to reliably report the presence or absence of toxicological compounds of interest, easily streamline workflows, and increase the speed of analysis for complex matrices using the scientific library functionality and flexible reporting templates.Capture, process and analyze complex high-end mass spectrometry and chromatography data on a single software platform. Gain the ability to test and confirm the presence of compounds in a single measurement. Drastically increase workflow efficiency and improve data review and reporting time with pre-defined workflows. Transform your laboratory with the most innovative screening solution ever built for forensic toxicology testing.Forensic Toxicology Screening Solution with UNIFI Features: Reliably detect and report the presence of drugs and other compounds of interest, while minimizing both false positive and negative results Identify known and unknown compounds with the UNIFI Toxicology Scientific Library Build a historical record of sample analysis data for retrospective review as new challenges emerge Ensure compliance with best-in-class data security and traceability
(0)
ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
Waters
ACQUITY UPLC® HSS (High Strength Silica) T3 Columns contain the first and only 100% silica particle designed, tested and intended for use in applications up to 15000 psi/1000 bar. The ACQUITY UPLC® HSS particle is not an HPLC particle. High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high column packing and operating pressures of UPLC® separations. The first ligand chemistry in this UPLC®-certified column family utilizes T3 bonding in order to retain and separate polar organic compounds. ACQUITY UPLC® HSS T3 columns possess the superior polar-compound retention, aqueous mobile phase compatibility and ultra-low MS bleed of Atlantis® T3 columns. Easier Method Transfer Since Atlantis® T3 HPLC and ACQUITY UPLC® HSS T3 columns utilize T3 bonding, LC methods can be transferred from HPLC to UPLC® separations. Because Atlantis® and HSS particles are different substrates, some method modification and/or optimization may be required. A compelling benefit of UPLC® technology is the ease and speed at which this method optimization can occur since UPLC® separations offer greater resolution in less time. Moving from XBridge™ HPLC to ACQUITY UPLC® BEH columns is nearly seamless, as the hybrid particle substrates differ only in particle size. However, because of T3 bonding it should be straightforward to make simple adjustments to successfully transfer a separation from an Atlantis® T3 HPLC column to an ACQUITY UPLC® HSS T3 column.
(13)